柑桔炭疽菌果胶裂解酶pelB基因特异性引物的筛选  

Screening of pelB-specific primers for pectate lyase gene of Colletotrichum gloeosprioides

作　　者：谭萍[1] 王勇[1] 姜于兰[1] 

机构地区：[1]贵州大学农学院,贵州贵阳550025

出　　处：《广东农业科学》2012年第1期130-131,135,共3页Guangdong Agricultural Sciences

基　　金：贵州省农业攻关项目(黔科合NY字[2009]3006)

摘　　要：以柑桔炭疽菌为主要研究对象,根据已知的炭疽菌果胶裂解酶pelB基因序列,运用GenBank及Primer Premier 5共设计6对引物,其中编号为PB1/PB2、Cg1A/Cg1b及Cg2a/Cg2b的引物可扩增出pelB基因片段,经同源性分析,全序列与GenBank中登录号为GU478342.1 pelB基因的的同源性高达98%,PB1/PB2、Cg1A/Cg1b对柑桔胶孢炭疽菌果胶裂解酶pelB基因有较高特异性。Colletotrichum gloeosprioides was the main object in this study.Six pairs of primers according to the conservative sequence of the reported pelB gene were designed with GenBank and Primer Premier 5 and then the six pairs of primers were amplified by PCR.The result showed that the primers numbered PB1/PB2,Cg1A/Cg1b and Cg2a/Cg2b could be amplified pelB gene fragment.Comparing and analysising the sequencing result to the reported sequence showed that the amplified fragment of pelB gene was above 98% homology with GenBank the accessioned number GU478342.1.However,the three specific designed primers were not specific with other common pathogens.

关 键 词：胶孢炭疽菌 果胶裂解酶 特异性引物 

分 类 号：S436.661.14[农业科学—农业昆虫与害虫防治]