血管紧张素Ⅱ对小鼠足细胞小窝蛋白1表达的影响  

Role of caveolin-1 in angiotensin Ⅱ-induced podocyte injury in vitro

作　　者：徐婉[1] 任志龙[1] 梁伟[1] 陈铖[1] 陈星华[1] 丁国华[1] 

机构地区：[1]武汉大学人民医院肾内科,武汉430060

出　　处：《临床肾脏病杂志》2012年第1期37-40,共4页Journal Of Clinical Nephrology

基　　金：国家自然科学基金资助项目（编号：30871167,30900688）

摘　　要：目的 观察血管紧张素Ⅱ（AngⅡ）刺激及氯沙坦干预对足细胞小窝蛋白1（caveolin-1）表达的影响,探讨caveolin-1在足细胞损伤中的作用.方法 体外培养永生化小鼠足细胞（MPC）,AngⅡ（10-6mol/L）刺激不同时间（3 h、6h、12 h和24 h）；Losartan（10-6 mol/L）提前预处理3h后与AngⅡ（10-6 mol/L）共孵育6h,Hoechst-33342检测足细胞凋亡率,Western-blot法检测各组细胞caveolin-1蛋白表达,免疫荧光检测caveolin-1及其磷酸化水平.结果 ①AngⅡ刺激3h,足细胞即开始发生凋亡,随着刺激时间的延长,足细胞凋亡明显增多（P＜0.05）.②AngⅡ刺激不同时间caveolin-1表达总量无明显改变（P＞0.05）,从3h开始,caveolin-1磷酸化水平明显增高（P＜0.05）.③Losartan干预后caveolin-1磷酸化水平明显降低（P＜0.05）.结论 caveolin-1可能在AngⅡ诱导的足细胞损伤中发挥着重要的作用.Objective To evaluate the possible role of caveolin-1 in angiotensin Ⅱ （Ang Ⅱ ）-induced podocyte damage. Methods Immortalized mouse podocytes were cultured in vitro. Podocytes were exposed to AngⅡ（10-6 mol/L） for different time lengths （3, 6, 12, and 24 h） with or without pretreatment of Losartan （10 ^-6 mol/L）. Apoptosis was evaluated by cell nucleus staining. Total proteins were prepared and evaluated for the expression of caveolin-1 by Western-blot analysis; Caveohn-1 and p-caveolin-1 expression was detected by using immunofluorescence. Results （1） Ang H induced podocyte apoptosis in a time-dependent manner （P〈0. 05） ; （2） The expression of caveolin-1 protein had no significant change after AngⅡ stimulation （P〉0. 05）, but the phosphorylation level of caveolind was enhanced greatly after exposure to Ang Ⅱ for 3 h, which was decreased significantly by pretreatment with Losartan （P〈0. 05）. Conclusions Caveolin-1 may play an important role in AngⅡ-induced podocyte damage.

关 键 词：血管紧张素Ⅱ 蛋白 足细胞 大鼠 

分 类 号：R692[医药卫生—泌尿科学]