高效液相色谱法测定丹红片中丹参素和原儿茶醛的含量  

Determination of Danshensu and Protocatechuic Aldehyde in Danhong Tablets by HPLC

作　　者：赵娅柯[1] 张长弓[1] 隆清娥[1] 张梦洁[1] 王腾[1] 

机构地区：[1]华中科技大学同济医学院药学院生药系,武汉430030

出　　处：《中国药师》2012年第1期68-70,共3页China Pharmacist

摘　　要：目的:建立丹红片中丹参素和原儿茶醛的含量测定方法。方法:采用高效液相色谱法,色谱柱为Diamonsil^(TM)C_(18)(250 mm×4.6 mm,5μm),以甲醇-0.05%磷酸水溶液(20:80)为流动相,检测波长为280 nm,流速为1.0 ml·min^(-1),柱温:30℃。结果:丹参素钠在8.160～97.920 mg·L^(-1)的浓度范围内与其峰面积呈良好的线性关系(r=0.999 3),平均回收率为100.06%,RSD=0.46%(n=6);原儿茶醛在0.292～2.336 mg·L^(-1)范围内具有良好的线性关系(r=0.999 1),平均回收率为99.92%,RSD=0.69%(n=6)。结论:该方法简便易行,重复性好,准确度高,为丹红片的质量控制奠定了基础。Objective： To develop an HPLC method for the determination of Danshensu and protocatechuic aldehyde in Danhong tablets. Method： A DiamonsilTM Cls column （250 mm x4. 6 mm,5 um） was used at 30 ℃ ,the mobile phase consisted of methanol- 0. 05% phosphoric acid aqueous solution （20： 80） ,the flow rate was 1.0 ml-min-1 and the detection wavelength was 280 nm. Result： The tinear ranges were 8. 160-97. 920 mg.L-1 （r =0. 999 3） for sodium Danshensu and 0. 292-2. 336 mg-L-1 （r =0. 999 3）for proto- eatechuic aldehyde. The average recovery of Danshensu and protocatechuie aldehyde was 100. 06% （ RSD = 0. 46% ） and 99. 92% （RSD = 0. 69% ）,respectively （n = 6 ）. Conclusion： The method is simple, accurate and reliable with good reproducibility. It can be used as a quality control method for Danhong tablets.

关 键 词：丹红片 丹参素 原儿茶醛 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]