早期戒烟大鼠肺病理及炎性介质变化  

Changes in Lung Pathology and Expression of Inflammatory Mediators after Smoking Cessation Early in Rats

作　　者：姜洪芳[1] 蔡存伟[2] 赵立[3] 

机构地区：[1]中国医科大学附属盛京医院老年病科,沈阳110004 [2]辽宁省肿瘤医院病理科,沈阳110042 [3]中国医科大学附属盛京医院呼吸内科,沈阳110004

出　　处：《中国比较医学杂志》2012年第1期51-53,61,83,共5页Chinese Journal of Comparative Medicine

基　　金：辽宁省自然科学基金资助项目(20092103)

摘　　要：目的观察早期戒烟后大鼠肺组织病理及炎性介质表达变化规律。方法选用Wistar雄性大鼠80只,随机分为对照组及早期戒烟后0天、1周、2周、4周、6周、8周、12周组。采用酶联免疫吸附方法测定各组大鼠血清中IL-8的蛋白质含量,S-P免疫组化学方法检测肺组织NF-κB p65的表达,并光镜下观察HE染色切片、对大鼠气道炎症进行病理学评分。结果早期戒烟组大鼠可见气道上皮细胞纤毛发生粘连、倒伏,上皮细胞空泡变形、坏死、增生,炎症细胞浸润;其血清IL-8浓度、肺组织NF-κB的表达及气道炎症病理评分在戒烟后各时相点较未吸烟对照组明显升高,有统计学意义(P<0.05)。早期戒烟组大鼠血清IL-8的浓度、肺组织NF-κB的表达及肺组织病理炎症评分在戒烟后略有上升、且在戒烟后8周达到高峰,但随后在戒烟12周时可见IL-8的浓度有下降趋势,肺组织病理炎症反应有所减轻。结论早期戒烟大鼠在戒烟早期虽可见炎症反应略有加重,但随戒烟时间延长,仍可见炎症反应有所减轻。因此,提倡及早且坚持戒烟。Objective To investigate changes in lung pathology and expression of inflammatory mediators in early quitting smoke rats.Methods A total of 80 Wistar male rats were randomly divided into control group and early quitting smoke groups on the smoking cessation stage 0 day,1 week,2 weeks,4 weeks,6 weeks,8 weeks,12 weeks.Levels of IL-8 protein in serum of different group rats were detected by enzyme-linked immunosorbent assay（ELISA）,expressions of NF-κB p65 in lung tissue were detected by S-P immunohistochemistry,and pathology score of inflammation on airway were carried out by observing HE stained lung tissue sections under light microscope.Results Early quitting smoke group could be seen Cilia sticking together,lodging,epithelial cell vacuoles deformation,necrosis,hyperplasia,inflammatory cell infiltration.The levels of IL-8 protein in serum,expression of NF-κB and pathological score in lung tissue of smoking cessation groups on any smoking cessation stage was significantly higher than non-smoking control group,statistically significant（P0.05）.In early quitting smoke groups,the serum level of IL-8,expression of NF-κB and airway inflammation pathological score of groups was the highest after 8 weeks of smoking cessation,and then the serum level of IL-8 tended to decrease and the lung inflammation alleviated on the smoking cessation stage 12 weeks.Conclusion Although on the early stage of smoking cessation,inflammatory response can be seen a slight increase,but with the cessation prolonged inflammatory response can still be seen some relief.Therefore,it is necessary to persist on quitting smoking as soon as possible.

关 键 词：早期戒烟 肺组织病理 炎性介质 

分 类 号：R563.3[医药卫生—呼吸系统] R332[医药卫生—内科学]