大鼠完全脊髓横断损伤膀胱功能障碍模型的建立及评价  

Evaluation of a rat model of bladder dysfunction induced by complete spinal cord transection

作　　者：赵雪燕[1] 石萍[1] 孙煜浩[2] 蓝宁[1] 

机构地区：[1]上海交通大学Med-X研究院,上海200030 [2]上海交通大学医学院附属瑞金医院

出　　处：《中国康复医学杂志》2012年第2期99-102,共4页Chinese Journal of Rehabilitation Medicine

基　　金：中国博士后科学基金(20100480601)

摘　　要：目的:建立和标准化一个骶髓上完全脊髓横断损伤引起的膀胱功能障碍的大鼠模型。方法:在16只成年雌性Sprague-Dawley大鼠脊髓T9—T11处进行椎板切除,造成完全脊髓横断。采用术后每天手动按摩膀胱排尿的尿量变化和脊髓损伤大鼠与健康大鼠的两组膀胱内压图比较来进行评价。6只鼠作为健康对照。结果:通过术后护理降低并发症。术后每天进行按摩排尿,尿量不断上升,1周左右达到峰值,之后开始下降。对健康大鼠和脊髓损伤大鼠的膀胱以相同灌注速度持续灌注生理盐水,正常大鼠的膀胱呈现稳定的周期性排尿收缩活动,而脊髓损伤大鼠在膀胱内压持续升高后产生重复性强烈的排尿收缩。结论:在脊髓休克期结束之后的脊髓恢复期内,所有脊髓损伤模型的大鼠膀胱都呈现逼尿肌—括约肌协同失调症状。本实验成功地建立了大鼠完全性脊髓损伤引起的膀胱功能障碍的模型,此模型简单经济,为进一步研究脊髓损伤下膀胱功能障碍的电刺激调节和康复提供了有价值的大鼠模型。Objective： To establish and evaluate bladder dysfunction following complete suprasacral spinal cord transection in a standardized rat model. Method： Sixteen adult female Sprague-Dawley rats were operated at Tg--Tll vertebra, after laminectomy rat＇s su- prasacral spinal cords were completely transected. After surgery daily weight of expressed urine was measured and recorded to assess the changes of bladder activity. Measurements of continuous uroflow cystometry were performed and compared between intact rats and spinal cord transected rats. Six healthy rats were used as controls. Result： Most of the complications could be relieved with post-surgery care. The weight of urine increased initial- ly and reached to a peak at 1-week post-spinal-transection followed by gradually declining. Under continuous in- fusion of saline into the bladder with constant rate, the bladder of intact rats initiated periodic voiding contrac- tions. In contrast,the bladder of spinal-transection rats showed uninhibited non-voiding contractions with increasing pressure and smaller void contractions at larger bladder volumes with high and sustained intravesical pressures. Conclusion： In the recovery period the spinal cord transected rats had detrusor-external sphincter dyssynergia. The rat model with bladder dysfunction following complete suprasacra] spinal cord transection was successfully es- tablished. The rat model could be used to investigate the efficacy of sacral-neuromodulation for treating bladder dysfunction in future studies. Author＇s address Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030

关 键 词：脊髓损伤 膀胱功能障碍 大鼠模型 神经调节 

分 类 号：R651.2[医药卫生—外科学] R694[医药卫生—临床医学]