乙型肝炎疫苗联合B7-H1蛋白疫苗对HBV转基因小鼠抗HBsAg免疫应答的影响  

Immune response to HBsAg can not be enhanced with B7-H1 protein vaccine

作　　者：苏雯静[1] 张野[1] 刘博[1] 连建奇[1] 黄长形[1] 白雪帆[1] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心

出　　处：《细胞与分子免疫学杂志》2012年第2期113-116,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家"十一五"传染病重大专项资助(2008ZX10002-008)

摘　　要：目的:研究B7-H1蛋白疫苗对HBV转基因小鼠免疫应答的影响,探索治疗慢性乙型肝炎的新方法。方法:用不同剂量的乙型肝炎疫苗和B7-H1蛋白疫苗联合免疫HBV转基因小鼠,应用ELISA法检测转基因小鼠在不同时间点血清抗B7-H1抗体滴度,同时在免疫后第14周末处死小鼠取脾细胞,检测不同的免疫方法对小鼠脾细胞产生HBsAg特异性Th1类细胞因子(IFN-γ及IL-2)、对HBsAg特异性分泌IFN-γT细胞数量及对小鼠淋巴细胞增殖的影响。结果:成功完成小鼠的免疫计划,5周起血清中即能测到B7-H1抗体,同一时间点各组之间的抗体滴度值并无明显差异。加B7-H1蛋白免疫各组与相同剂量单用HBsAg蛋白免疫各组相比:IL-2均明显减低(P<0.05),分泌IFN-γT的T细胞数量下降,但脾淋巴细胞分泌IFN-γ的水平各组间无明显差异;MTT法测定的淋巴细胞增殖能力各组间也无明显变化。结论:B7-H1蛋白疫苗可诱导HBV转基因小鼠产生明显的抗B7-H1抗体应答,但不能增强抗HBsAg的免疫应答。较小剂量的HBsAg即可引起HBV转基因小鼠Th1类细胞因子(IFN-γ及IL-2)的分泌以及淋巴细胞增殖。AIM： To observe the immunization effect to HBsAg by B7-H1 protein vaccine in transgenic mice,and to explore new methods for the treatment of chronic hepatitis B.METHODS： After the joint immunization in HBV transgenic mice with different doses of hepatitis B virus（HBV） vaccine and B7-H1 protein,the anti-B7-H1 antibody titors,Th1 type of cytokines（IFN-γ and IL-2） from the spleen cells of mice,the number of theT cells secreting IFN-γ and the number of the mice lymphocyte proliferation were mesrued by ELISA,ELISPOT and MTT technique respectively,to compare the immune effect of different immune methods and regimen.RESULTS： The immune plans were completed successfully.The anti-B7-H1 antibody was detected in the fifth week after immunization with B7-H1 vaccine,at the same time no obvious difference of antibodies titors between groups were found.IL-2 and the number of T cells secreting IFN-γ were significantly reduced（P0.05）in joint immunization group with B7-H1 protein and HBsAg,but no difference in other immune tests,such as IFN-γ,lymphocyte proliferation.CONCLUSION： A lower doses of HBsAg can cause the secretion of Th1 type of cytokines and lymphocyte proliferation.B7-H1 protein vaccines have a better immunogetic effect for HBV transgenic mice,but can notupregulate the immune response to HBsAg.

关 键 词：乙型肝炎病毒 转基因小鼠 细胞因子 程序性死亡因子1受体1 淋巴细胞增殖 

分 类 号：R512.62[医药卫生—内科学]