Wister大鼠心肌缺血预调置与一氧化氮相关性的动物实验研究  

Animal experimental study in Wister rat′s ischemic myocardia preconditioning with NO relativity

作　　者：孙永波[1] 谭学厚[1] 

机构地区：[1]淄博职业学院生理免疫室,山东淄博255314

出　　处：《山东大学学报（医学版）》2012年第1期42-45,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨一氧化氮(NO)与心肌缺血预调置作用的相关性。方法将50只体质量300～350 g雄性健康Wistar大鼠分为对照组、缺血/再灌注损伤(I/R)组、预调置组、L-硝基精氨酸(L-NNA组)和L-精氨酸(L-Arg)+L-NNA等5组,分别观察冠脉灌注压(CPP)和心肌蛋白、乳酸脱氢酶(LDH)、环一磷酸鸟苷(cGMP)和丙二醛(MDA)含量等心肌损伤指标的变化。结果与I/R组比较,预调置组心肌蛋白漏出量明显降低、LDH漏出量显著下降、心肌MDA含量明显降低、cGMP水平则明显升高(P均<0.01),CPP降低(P<0.05);L-NNA灌流组可以阻断预调置的心肌保护作用,L-NNA+L-Arg灌流组可逆转L-NNA阻抑预调置的心肌保护作用。结论心肌缺血预调置明显提高缺血心肌对缺血缺氧的耐受力,与改善心肌I/R损伤时NO缺陷密切相关。Objective To discuss the relation of NO to the ischemic myocardia preconditioning function.Methods 300-350 g male health Wistar rats were divided into the control,I/R,preconditioning,L-NNA and L-Arg＋L-NNA groups,to observe the variation of CPP and myocardial protein leaks,LDH,and cGMP as well as MDA contents of myocardial injured index.Results Compared with the I/R group,myocardial protein leaks quantity was obviously reduced,LDH leaks quantity was obviously decreased,myocardial MDA content was obviously reduced,and cGMP level was obviously elevated,All had statistical significance（P0.01）.CPP was reduced（P0.05）.L-NNA perfusion may interdict myocardial protection of preconditioning,L-NNA＋L-Arg perfusion can reverse myocardial protection of preconditioning interdicted by L-NNA perfusion.Conclusion Ischemic preconditioning obviously enhances ischemic myocardia tolerance to ischemia and anoxia,and it is closely related to improve ment of NO deficiency in myocardial I/R injury.

关 键 词：缺血预调置 缺血/再灌注损伤 心肌缺血 一氧化氮 

分 类 号：R541.4[医药卫生—心血管疾病] R-332[医药卫生—内科学]