肺结核患者杀伤细胞免疫球蛋白样受体基因多态性分析  

Polymorphisms of killer cell immunoglobulin-like receptor genes in patients with pulmonary tuberculosis

作　　者：路超[1] 沈亚娟[1] 邓云峰[2] 王长印[1] 张炳昌[1] 范刚[1] 赵跃然[3] 刘义庆[1] 赵胜梅[1] 王子娥[1] 张春芝[1] 卢志明[1] 

机构地区：[1]山东大学附属省立医院检验科,济南250021 [2]山东省胸科医院感染办公室,济南250013 [3]山东大学附属省立医院科学研究中心,济南250021

出　　处：《山东大学学报（医学版）》2012年第1期122-128,共7页Journal of Shandong University:Health Sciences

基　　金：山东省科学技术攻关基金资助项目(2009GG10002014)

摘　　要：目的探讨杀伤细胞免疫球蛋白样受体(KIRs)基因多态性与肺结核(TB)的关系。方法采用序列特异性引物聚合酶链反应(SSP-PCR),分析109例肺结核患者(TB组)和30例健康者(对照组)中14个KIR基因多态性。TB组又分为30例痰涂片抗酸杆菌阳性(SP组)和79例涂片阴性患者(SN组)。结果与对照组比较,SP组KIR2DL5(P=0.007)、2DS1(P=0.038)、2DS3(P=0.010)、3DS1(P=0.002)阳性率增高,KIR2DS5频率降低(P=0.038);SN组KIR2DL5(P=0.018)、2DS1(P=0.000)、2DS5(P=0.000)、3DS1(P=0.001)阳性率增高;KIR2DS4(P=0.004)和KIR2DS5(P=0.000)频率SP组较SN组升高;患者携带两个及以上活化KIR的频率较对照组明显升高(P=0.013);患者组基因型AJ的频率较对照组显著降低。结论 KIR基因多态性可能与肺结核的易感性有关。Objective To investigate association of polymorphisms of killer cell immunoglobulin-like receptor（KIR） genes with susceptibility to tuberculosis（TB） infection.Methods The sequence specific primer polymerase chain reaction（SSP-PCR） method was used to analyze 14 KIR genes in genomic DNA in 30 smear-positive（SP） patients,79 smear-negative（SN） patients and 30 healthy controls.Results Frequencies of KIR2DL5（P=0.007）,2DS1（P=0.038）,2DS3（P=0.010） and 3DS1（P=0.002） were statistically significantly higher in the SP group than in the control group,while KIR2DS5（P=0.038） was lower.Also,frequencies of KIR2DL5（P=0.018）,2DS1（P=0.000）,2DS5（P=0.000） and 3DS1（P=0.001） were significantly higher in the SN group than in the control group.Compared with the SN group,frequencies of KIR2DS4（P=0.004） and KIR2DS5（P=0.000） were higher in the SP group.In addition,the number of subjects carrying more than two activating KIR genes in the TB group was higher than that in the control group（P=0.013）.The frequency of genotype AJ was lower in TB patients than in the controls.Conclusion Polymorphisms of KIR genes may be associated with susceptibility to TB infection.

关 键 词：杀伤细胞免疫球蛋白样受体 结核 肺 多态性 单核苷酸 

分 类 号：R521[医药卫生—内科学]