即时浸酸显著提高滞育性家蚕卵辅酶Ⅰ和Ⅱ含量  

Instant hydrochloride acid soaking enhances NAD(H) and NADP(H) levels in diapausing eggs of the silkworm,Bombyx mori

作　　者：姚金美[1] 万华星[1] 司马杨虎[1] 赵林川[1] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123

出　　处：《昆虫学报》2012年第1期24-28,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31072090);现代农业产业技术体系(CARS-22-ZJ0104)

摘　　要：即时浸酸在阻止家蚕Bombyx mori卵滞育发动的同时,提高了其呼吸耗氧量,抑制了山梨醇积累。本研究利用HPLC法测定了家蚕滞育卵和5min即时浸酸滞育性卵中辅酶Ⅰ和Ⅱ含量。结果表明:产下后24-72h,家蚕滞育卵中NAD,NADH,NADP和NADPH含量分别下降了30%,37%,50%和4%;而即时浸酸滞育性卵中分别增加了77%,46%,142%和241%。不过,即时浸酸并未显著改变滞育性家蚕卵中NADH/NAD和NADPH/NADP比值。据此推测,即时浸酸提高滞育性家蚕卵辅酶Ⅰ含量与其呼吸耗氧量增加有关;即时浸酸显著提高辅酶Ⅱ含量与山梨醇积累抑制无关,而主要与生物合成加强有关。For diapausing eggs of the silkworm,Bombyx mori,diapause initiation is prevented with instant hydrochloric acid（HCl） soaking,which can cause the marked enhancement of oxygen consumption and the inhibition of sorbitol accumulation.NAD（H） and NADP（H） levels in diapause eggs and those soaked with HCl for 5 min at 24 h after oviposition were determined using HPLC methods.From 24 h to 72 h after oviposition,levels of NAD,NADH,NADP and NADPH in diapausing eggs decreased by 30%,37%,50% and 4%,respectively,whereas those in diapausing eggs soaked with HCl increased by 77%,46%,142% and 241%,respectively.Moreover,no significant effect of HCl on both NADH/NAD and NADPH/NADP ratios was observed.It is so inferred that,in diapausing eggs soaked with HCl,the marked increase of NAD（H） is involved in the marked enhancement of oxygen consumption,whereas the enhancement of NADP（H） is related to the stronger biosynthesis rather than the inhibition of sorbitol accumulation.

关 键 词：家蚕 滞育 滞育卵 即时浸酸 辅酶Ⅰ 辅酶Ⅱ 

分 类 号：Q966[生物学—昆虫学]