Bcl-2/Bax蛋白表达对皮瓣重建兔阴囊生精细胞凋亡的影响  

Bcl-2/Bax Expression and Spermatogenic Cells Apoptosis after Scrotal Reconstruction with Skin Flap of Rabbit

作　　者：崔红旺[1,2] 王达利[1] 孙广峰[1] 魏在荣[1] 

机构地区：[1]遵义医学院附属医院整形外科,遵义563003 [2]新乡医学院第一附属医院手外科,新乡453100

出　　处：《生殖与避孕》2012年第2期87-91,共5页Reproduction and Contraception

基　　金：贵州省优秀科技教育人才省长专项基金项目;项目号:2005-65

摘　　要：目的:探讨皮瓣重建兔阴囊生精细胞的凋亡与Bcl-2/Bax蛋白表达的关系。方法:将12只育龄期健康雄性新西兰大白兔随机分成实验组和对照组,每组6只。采用温度计埋藏法测量实验组皮瓣重建前阴囊内睾丸表面温度,手术切除实验组动物阴囊,建立皮瓣重建阴囊动物模型,对照组不作处理。动物模型建立后第8周末,实验组动物测量睾丸表面温度后行睾丸活检;对照组直接进行睾丸活检。采用原位末端标记(TUNEL)法检测睾丸生精细胞凋亡指数,免疫组织化学法结合图象分析仪检测睾丸生精细胞Bcl-2/Bax蛋白的表达。结果:实验组模型建立前睾丸表面温度为36.0±0.3℃,模型建立后第8周末睾丸表面温度为38.1±0.6℃(P<0.05)。模型建立后第8周末实验组睾丸生精小管中生精细胞的凋亡指数(AI)为71.85±2.69%,对照组AI为7.73±4.95%(P<0.05)。手术使Bax表达显著升高(P<0.05),Bcl-2表达显著降低(P<0.05);凋亡细胞主要为初级精母细胞和圆形精子细胞。结论:皮瓣重建兔阴囊后睾丸局部温度升高,诱导睾丸生精细胞凋亡增加;生精细胞凋亡增加与Bcl-2基因表达降低、Bax基因表达升高密切相关。细胞内Bcl-2/Bax比值是影响生精细胞凋亡的重要因素之一。Objective： To investigate the relationship of apoptosis of spermatogenous cell and the expression of Bcl-2 and Bax after scrotal reconstruction with skin flap.Methods： Twelve males healthy NewZealand white rabbits were included and equally randomized into experimental and control groups.The scrota of the experimental rabbits were excised and reconstructed with the hypogastric flap,while the control rabbits were left untreated.At the end of the 8th week after surgery,6 animals were randomly taken from each of the two groups form measurement of the testis surface temperature and testicular biopsy.The apoptosis of spermatogenous cells in the testis tissues was detected by TUNEL assay,and the expression of Bcl-2 and Bax were determined by immunohistochemistry and imaging analysis.Results： At the end of the 8th week after scrotal reconstruction,the testicular surface temperature was 38.1 ± 0.6℃ in experimental group,significantly higher than 36.0 ± 0.3℃ before surgery（P0.05）,and the apoptosis index（AI） of the spermatogenous cells was 71.85 ± 2.70%,as compared with 7.73 ± 4.95% in the control（P0.05）.In the testes of the scrotum reconstruction group,Bax’s epression significantly increased compared with the control,respectively（P0.05）,while Bcl-2’s expression obviously decreased（P0.05）.The apoptotic cells were mostly primary spermatocytes and round spermatides.Conclusion： The testicular surface temperature rises after scrotal reconstruction with the hypogastric flap,which increases the apoptosis of spermatogenic cells.The spermatogenic cell apoptosis is highly correlated with the expressions of Bcl-2 and Bax in testiculus spermatogenic cells after scrotum reconstruction with flaps.Bcl-2/Bax plays an important role in spermatogenic cells apoptosis.

关 键 词：重建阴囊 皮瓣 生精细胞 凋亡 BCL-2/BAX 兔 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] R697.2[医药卫生—基础医学]