血管生长因子受体3(VEGFR3)在胎盘的细胞定位及其在ART安全性研究中的意义  

Location and Expression of VEGFR3 in Placenta and the Value of VEGFR3 in the Safety Study of ART

作　　者：汤丽莎[1] 张园[2] 赵海君[2] 骆秀翠[1] 高莉[2] 崔毓桂[2] 刘嘉茵[2] 

机构地区：[1]连云港市妇幼保健院生殖医学中心,连云港222006 [2]南京医科大学第一附属医院生殖医学科,南京210029

出　　处：《生殖与避孕》2012年第2期135-138,共4页Reproduction and Contraception

基　　金：连云港市卫生局科教兴卫指令性重点人才课题(ZC233);973项目(2006CB944005)

摘　　要：目的:探讨血管生长因子受体3(vascular endothelial growth factor 3,VEGFR3)在ART妊娠胎盘组织中的差异表达及其安全性研究中的意义。方法:收集11例自然妊娠(对照组)和10例ART妊娠(ART组)的单胎足月妊娠选择性剖宫产分娩的胎盘组织,分析比较组间的临床资料;并应用免疫组织化学技术和实时荧光定量PCR技术,观察VEGFR3在胎盘组织的细胞定位,比较VEGFR3在ART和对照组胎盘组织中的差异表达。结果:VEGFR3在胎盘组织中定位于胎盘绒毛合体滋养层细胞胞质。与对照组相比,VEGFR3细胞定位一致,ART妊娠的胎盘组织中VEGFR3 mRNA表达水平有上升趋势,但无统计学差异(P>0.05)。结论:ART妊娠的胎盘组织中VEGFR3细胞定位和表达水平未见明显变化。Objective： To investigate the research value of vascular endothelial growth factor 3（VEGFR3） in the safety study of assisted reproductive technology（ART）,the location and expression of VEGFR3 in placentae.Methods： Placentae were collected from full-term singletons both from ART pregnancies（n=10,ART group） and natural pregnancies（n=11,control group）,clinical data of all cases were analyzed;Immunohistochemistry and real time PCR were applied to study the location and expression of VEGFR3 in placentae.Results： VEGFR3 was expressed and localized in the cytoplasm of syncytiotrophoblast cells in human placenta.Compared with the control,VEGFR3 expression was up-regulated,but there was no significant difference,P0.05.Conclusion： There is no variation in location and expression of VEGFR3 in placenta from ART.

关 键 词：辅助生殖技术(ART) ART安全性 胎盘 血管生成 血管生长因子受体3(VEGFR3) 

分 类 号：R714[医药卫生—妇产科学]