HPLC测定附子与“半蒌贝蔹及”各药材配伍前后3种双酯型生物碱的含量变化  

Determination of Content Changes of Three Diester-diterpenoid Alkaloids in Compatibility of Radix Aconiti Laterlis Preparata and Rhizoma Pinelliae,Fructus Trichosanthis, Bulbus Fritillariae,Radix Ampelopsis,Rhizoma Bletillae by HPLC

作　　者：董欣[1,2] 王淑敏[1] 李晓明[1] 宋凤瑞[2] 刘志强[2] 刘淑莹[2] 

机构地区：[1]长春中医药大学,吉林长春130117 [2]中国科学院长春应用化学研究所,吉林长春130022

出　　处：《中药材》2011年第12期1891-1894,共4页Journal of Chinese Medicinal Materials

基　　金："973"计划中医理论专项(2006CB504706);国家自然科学基金(30772721)

摘　　要：目的:应用现代分析检测技术,定量分析附子与半夏、瓜蒌、贝母、白蔹、白及配伍前后水煎液中3种双酯型生物碱(乌头碱、中乌头碱、次乌头碱),考察其含量变化的影响因素。方法:采用高效液相色谱法,Agilent Ex-tend C18柱,乙腈-甲醇(V∶V=1∶1)-35 mmol/L乙酸铵梯度洗脱,流速0.6 mL/min,检测波长235 nm。结果:除川贝母、白蔹、法半夏外,其他各药与附子配伍后煎煮液中3种双酯型生物碱均高于附子单煎液,而其变化趋势与煎煮液的pH值有联系。结论:共煎液的pH值是影响药液中3种双酯型生物碱含量的直接因素。Objective： To analyze the three diester-alkaloids content in the decoctions before and after Radix aconiti lateralis preparata combined with Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae,Radix ampelopsis,Rhizoma bletillae,respectively.Methods： HPLC analysis was performed on a Agilent Extend C18 column,eluted with a mobile phase consisted of acetonitrile/ methanol-35 mmol/L ammonium acetate and gradient elution,with a flow rate of 0.6 mL/min and the detection wavelength of 235 nm.Results： The contents of three diester-alkaloids in the co-decoctions of Radix aconiti laterlis preparata combined with Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae,Rhizoma bletillae were more than that of single Radix aconiti laterlis preparata decoction,expect the Bulbus fritillariae cirrhosae,Radix ampelopsis and Rhizoma pinelliae praeparatum.From the result,we can found that the content of three diester-alkaloids in decoctions was correlated with the decoction pH.Conclusion： The pH of decoction is one of the most important factors to relate the three diester-alkaloids content in the decoctions.

关 键 词：附子 十八反 双酯型生物碱 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]