大鼠饲料对黄酮类成分药代动力学试验的干扰(英文)  

Study on the Interference of Rat Feed to Pharmacokinetics of Flavonoids

作　　者：杨翠平[1,2] 刘孟华[1,3] 邹威[1] 方思琪[1] 苏薇薇[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]军事医学科学院毒物药物研究所,北京100850 [3]广东环球制药有限公司,广东佛山528308

出　　处：《中药材》2011年第12期1906-1911,共6页Journal of Chinese Medicinal Materials

基　　金：国家“重大新药创制”科技重大专项(2011ZX09102-011-03)

摘　　要：目的:研究黄酮类成分药代动力学试验中,大鼠饲料对测定结果的干扰。方法:采用LC-ESI-MS/MS法对大鼠饲料和血浆中的黄酮进行分离,并根据其鉴定。结果:在大鼠标准饲料中检测到5个柑橘类黄酮(柚皮苷、柚皮素、橙皮苷、橙皮素和槲皮素)和2个大豆异黄酮(大豆素、大豆苷),同时在采用该饲料喂养的大鼠血浆中也检测到这7个黄酮。结论:在进行黄酮类成分的药代动力学试验时,饲料来源的黄酮会干扰血浆中该物质的测定,影响研究结果的准确性。Objective：To investigate the interference of rat feed to pharmacokinetic of flavonoids.Methods：Flavonoids in rat feed and plasma samples were separated by rapid resolution reversed-phase HPLC and characterized by LC-ESI-MS/MS analysis with an electrospray ionization source（ESI） and a triple quadrupole analyzer by fragmentation pattern.Results：Five Citrus flavonoids（naringin,naringenin,quercetin,hesperidin and hesperetin） and two soybean isoflavonoids（daizin,daidzein） were identified in common formula rat feed.The presence of these flavonoids in plasma from rats receiving the feed was also confirmed.Conclusion：The results showed the flavonoids in animal food might interfere with pharmacological and pharmacokinetic study of foreign natural compounds.This paper could be taken as a reference to pharmacological and pharmacokinetic studies of all the related substances.

关 键 词：黄酮 药代动力学 大鼠饲料 干扰 LC-ESI-MS/MS 

分 类 号：R285.5[医药卫生—中药学]