枸杞多糖对自然衰老雌性大鼠卵巢保护作用机制的探讨  

Discussion of Protective Mechanism of Lyceum barbarum Polysaccharides on Ovarian Tissue in Female Senile Rats

作　　者：韦敏[1,2] 郑生智[1,2] 马红[3] 吕晔[1,2] 

机构地区：[1]中国科学院江苏省植物研究所 [2]江苏省药用植物研究开发中心,江苏南京210014 [3]南京中医药大学,江苏南京210029

出　　处：《中药材》2011年第12期1915-1918,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然基金(30973927);江苏省中科院植物所青年基金(200802)

摘　　要：目的:研究枸杞多糖(Lyceum barbarum Polysaccharides,LBP)对自然衰老雌性大鼠雌二醇(E2)、孕酮(P)、IGF-Ⅰ、IGFR和IGFBP-1水平的影响,初步探讨枸杞多糖对自然衰老雌性大鼠作用的机制。方法:14月龄SD大鼠随机分为模型组、阳性对照组及枸杞多糖高、中、低剂量组,4月龄SD雌性大鼠为正常对照组。给药30 d后,取血及卵巢组织,放射免疫(RIA)法测定血清中雌二醇(E2)、孕酮(P)水平,酶免疫法测定血清中的IGF-Ⅰ与IGFR及卵巢组织中的IGFBP-1与IGFR水平。结果:卵巢功能衰退与激素(如雌激素、孕激素及IGF-Ⅰ)分泌能力呈正相关,而与IGFBP-1水平呈负相关。灌服不同剂量的LBP后能明显升高雌激素、孕激素及IGF-Ⅰ的水平,降低IGFBP-1水平,提示LBP能积极对抗卵巢功能的下降。结论:枸杞多糖对大鼠卵巢系统具有保护作用。Objective：To study the protective mechanism of Lyceum barbarum polysaccharides（LBP） on ovarian tissue in female senile rat.Methods：Senile rats of 14 months old were divided randomly into model group,positive drug group,LBP low dosage group,LBP medium dosage group and LBP high dosage group.SD female rats of 4 months old formed a youth group.30 days after administration,rat blood was taken,radioimmunoassay was employed to determine the levels of estrone and progesterone and enzyme immunoassay was used to determine the levels of IGF-Ⅰ.Then ovarian tissue was taken to determine the levels of IGFBP-1 and IGFR.Results：Daily oral LBP（60,40,20 mg/kg body weight）for 30 days significantly recovered uterine atrophy and restored serum IGF-Ⅰ level,estrone and progesterone levels that were decreased in elder rats,reduced the expressing of IGFBP-1 in ovarian tissue that were increased in elder rats.Conclusion：LBP has a notable protection on rat ovarian system.

关 键 词：枸杞多糖 自然衰老雌性大鼠 雌二醇 孕酮 IGF-Ⅰ IGFBP-1 IGFR 

分 类 号：R285.5[医药卫生—中药学]