大黄虫丸抗球囊损伤内皮诱导的家兔动脉粥样硬化作用机制  

Mechanism of Dahuang Zhechong Pill Against Atherosclerosis Induced by Balloon Angioplasty in Rabbits

作　　者：韩纯洁[1,2] 刘俊田[1] 张莹[1] 李明[1] 庞小明[1] 毛俊俊[1] 

机构地区：[1]西安交通大学医学院,陕西西安710061 [2]陕西省食品药品检验所,陕西西安710061

出　　处：《中药材》2011年第12期1919-1922,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助课题(30572347)

摘　　要：目的:观察大黄虫丸抗球囊损伤内皮诱导的家兔动脉粥样硬化的作用机制。方法:采用机械损伤动脉内皮辅以高脂饲料喂养的方法建立家兔动脉粥样硬化模型。将雄性新西兰家兔分为6组:正常对照组、假手术组、模型组、阳性对照组及大黄虫丸低、高剂量组。正常对照组、假手术组与模型组给予蒸馏水,阳性对照组给予丹参0.5 g/kg,大黄虫丸低、高剂量组分别给予大黄虫丸0.9、1.8 g/kg。灌胃给药,1次/d,连续60 d。实验结束时,用分光光度法测定血清丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)及血管壁髓过氧化物酶(MPO),SP免疫组织化学法测定血管平滑肌增殖细胞核抗原(PCNA)和BCL-2的表达。结果:大黄虫丸能降低血清MDA水平和血管壁MPO活性,升高SOD活性和NO含量,减少血管壁PCNA和BCL-2的表达。结论:大黄虫丸通过抗脂质过氧化作用、保护血管内皮、抑制VSMCs增殖和促进VSMCs凋亡等机制,抑制球囊损伤内皮诱导的动脉粥样硬化形成和发展。Objective：To study the mechanism of Dahuang Zhechong pill（DHZCP） against atherosclerosis induced by balloon angioplasty in rabbits.Methods：Atherosclerosis model was established by the combination of balloon angioplasty-induced endothelial injury and high cholesterol feeding in rabbit.Male New Zealand rabbits were divided into six groups randomly：normal control,sham,model,positive control and two doses of DHZCP-treated groups.Rabbits in DHZCP-treated groups were intragastrically administered 0.9 and 1.8 g/kg DHZCP for 60 days respectively,and rabbits in positive control group were given 0.5 g/kg Danshen.MDA,NO levels and SOD activity in serum,and MPO activity in the vascular wall were determined with spectrophotometry.Expressions of proliferating cell nuclear antigen（PCNA） and BCL-2 in the vascular wall were detected by SP immuohistochemical technique.Results：Compared with the model group,DHZCP significantly reduced serum MDA level and MPO activity in the vascular wall,increased serum NO level and SOD activity,and inhibited PCNA and BCL-2 expressions in the vascular wall.Conclusion：DHZCP inhibits the formation and development of atherosclerosis through anti-oxidative action,protecting endothelium from injury,inhibiting proliferation and promoting apoptosis of vascular smooth muscle cells.

关 键 词：大黄虫丸 动脉粥样硬化 内皮损伤 血管平滑肌增殖 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]