心房肽Ⅲ对离体大鼠心肌缺血再灌注后冠脉流量的影响  

Effects of Atriopeptide Ⅲ on coronary flow after ischemia-reperfusion of in isolated rat heart

作　　者：刘永欣[1] 孟存良[1] 张采红[1] 沙会然 王威静 

机构地区：[1]河北省人民医院心脏二科,河北石家庄050051 [2]河北省博野县医院内科,河北博野071300

出　　处：《河北中医》2011年第12期1863-1865,共3页Hebei Journal of Traditional Chinese Medicine

摘　　要：目的观察心房肽Ⅲ(APⅢ)对离体大鼠心肌缺血再灌注后冠脉流量的影响。方法将36只健康雄性SD大鼠腹腔内注射肝素,15 min后开胸取出心脏,立即置于0℃冷Krebs-Henseleit灌流液中,使其停跳。在液面下经主动脉断端行主动脉逆行插管,以线结扎固定,立即开始灌流。共选取36个心脏,随机分为正常对照组、模型对照组、阳性药对照组及APⅢ高、中、低剂量组6组,每组6个。阳性药对照组给予维拉帕米(浓度1×10-7 mol/L),APⅢ高、中、低剂量组APⅢ给药浓度分别为1×10-7、3×10-8、1×10-8 mol/L。除正常对照组持续灌流90 min外,其余各组均停灌30 min,复灌60 min。时测量冠脉流量(CF),计算CF恢复率。结果 APⅢ高、中、低剂量组和阳性药对照组均能促进再灌注后CF恢复。模型对照组复灌后各时间点CF恢复率均显著低于同时期正常对照组(P<0.01)。阳性药对照组各时间点CF恢复率均高于正常对照组(P<0.01,P<0.05);APⅢ高剂量组各时间点CF恢复率均高于模型对照组(P<0.01,P<0.05);APⅢ中剂量组除第10、50、60 min外均可显著增加CF恢复率(P<0.01,P<0.05);APⅢ低剂量组在第1、50、60 min显著增加CF恢复率(P<0.01,P<0.05);APⅢ高剂量组较阳性药对照组差异无统计学意义(P>0.05),APⅢ低剂量组和APⅢ中剂量组在1～20 min低于阳性药对照组。结论 APⅢ可以减少再灌注,"无复灌"现象,对离体心脏缺血再灌注后的冠脉具有保护作用。Objective To observe the effects of Atriopeptide Ⅲ on coronary flow after ischemia-reperfusion.Methods 36 healthy male SD rat received peritoneal injection of heparin.After 15 min the heart was removed by thoracotomy.Immediately placed in 0 ℃ cold perfusate of the K-H,made arrest.Under the surface the aortic retrograde was done and fixed by line and aortic ligation,and immediately began to perfusion.36 hearts were randomly divided into six groups,high,middle,low dosage of APⅢ（1×10-7mol/L,3×10-8mol/L and 1×10-8mol/L）group,verapamil group,control group and normal control group.The isolated rat heart was submitted to 30 min of on-perfusion which resulted in global ischemia,then 60 min of reperfusion.The coronary flows were measured.Results High,middle,low dosage of APⅢ group and verapamil group can promote the coronary flow recovery after reperfusion.The coronary flow recovery rates after reperfusion at different time in model group were lower than those in normal control group（P〈0.01）.The coronary flow recovery rates at different time in verapamil group were higher than those in normal control group（P〈0.01）.The coronary flow recovery rates at different time in high dose APⅢ group were higher than those in model group（P〈0.01）.The coronary flow recovery rates except at 10,50 and 60min in middle dose APⅢ group were obviously increased（P〈0.01,P〈0.05）.The coronary flow recovery rates at 1,50 and 60min in low dose APⅢ group were obviously increased（P〈0.01,P〈0.05）.There was no difference between verapamil group and high dose AP Ⅲ group on the coronary flow（P〈0.05）.The coronary flow in middle and low dosage APⅢ group are inferior to those in verapamil group.Conclusion APⅢ can attenuate the phenomena of ＂not reflow＂,which suggests that APⅢ has protective effect on cononary after the I/R in isdated rat heart.

关 键 词：心肌再灌注损伤 实验研究 疾病模型 动物 受体 心钠素 

分 类 号：R541.7[医药卫生—心血管疾病] R654.2[医药卫生—内科学]