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作 者:陈佳慧[1] 兰进好[1] 王晖[1] 王文文[1] 田纪春[2]
机构地区:[1]青岛农业大学农学与植物保护学院作物种质创新与品种改良实验室,山东青岛266109 [2]山东农业大学农学院小麦品质育种研究室/国家作物生物学重点实验室,山东泰安271018
出 处:《麦类作物学报》2011年第6期1001-1006,共6页Journal of Triticeae Crops
基 金:国家重点基础研究发展计划(973计划)项目(2009CB118301);山东省农业良种工程重大课题子课题(6208N1)
摘 要:为研究小麦籽粒形态及千粒重性状的QTL,以普通小麦6044和01-35为杂交组合构建的F8重组自交系(RIL)群体作为试验材料,在山东泰安(山东农业大学试验站)和莱阳(青岛农业大学试验站)两个环境下进行两年田间试验,利用Mapmaker/version 3.0和WinQTLCart软件通过复合区间作图法进行QTL初步定位,在两年两个环境下共检测到12个相关QTL位点,其中关于粒长的2个QTL分别位于2A和2B染色体上,可解释表型变异的25%和12%;4个粒厚QTL位于2A和6A染色体上,可解释表型变异的7%~10%;6个千粒重相关QTL位于染色体2A、4A和6A连锁群上,可解释表型变异的6%~25%;而粒宽QTL在两个地点上都没有检测到。其中相关性高的性状间有一些共同的QTL,表现出一因多效或紧密连锁效应。QTLs of kernel morphology and grain weight were investigated using a recombinant inbred line population derived from a cross between 6044×01-35 which were grown in Tai'an and Laiyang locations.Twelve QTLs for kernel morphology and 1000-grain weight were detected using Mapmaker/version 3.0 and WinQTL Cart based on composite interval mapping(CIM).Two QTLs for kernel length were detected on chromosomes 2A and 2B which could explain 25% and 12% of phenotypic variance;four kernel thickness QTLs were detected on chromosomes 2A and 6A across two environments,explaining 7%~10% of phenotypic variance;six 1000-grain weight QTLs were detected on chromosomes 2A,4A and 6A across two environments,explaining 6%~25% of phenotypic variance.There was no kernel width QTL detected in two environments.Some traits shared the same QTL,which was consistent with its high phenotypic correlations and showed tight linkages or pleiotropisms.
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