高效液相色谱法测定妇科消炎颗粒中芍药苷的含量  

Determination of paeoniflorin in Fukexiaoyan granula by HPLC

作　　者：马涵涛[1] 谢秀娟[1] 

机构地区：[1]河南科技大学第一附属医院,471003

出　　处：《中国现代药物应用》2012年第1期10-10,共1页Chinese Journal of Modern Drug Application

摘　　要：目的建立用高效液相色谱法测定妇科消炎颗粒中芍药苷含量的方法。方法采用色谱柱Shim-pack CLC-ODS(150mm×6mm),乙腈-水(16:65)为流动相,检测波长230nm,流速:1ml/min。结果妇科消炎颗粒中芍药苷在进样量0.2～1.2μg范围内呈良好的线性关系,r=0.9999,平均回收率为100.4%(n=6),RSD为1.6%。结论该方法快速准确、灵敏,为该制剂得质量控制建立了方法。Objective To establish a HPLC method for the determination of paeoniflorin in Fukexiaoyan granula.Methods The content of tetrahydropalmatine was determined by HPLC assay.The separation was performed on a Kromasil C18 column（150 mm×6 mm）with acetonitrile-water（16：65）as the mobile phase at the flow rate of 1 ml/min.The wave length was set at 230 nm.Results The linear range was 0.2～1.2 μg（r=0.9999）,while theaverage recovery was 100.4 %（n=6） with RSD of 1.6 %.Conclusion This method is simple,accurate and reliable,it can be a quality control standard of Fukexiaoyan granula.

关 键 词：妇科消炎颗粒 芍药苷 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]