生物肽-蛋氨酸脑啡肽增强树突状细胞抗肿瘤活性研究  

Bio-Peptide-Methionine Enkephalin(MENK) to Strengthen Antitumour Activity of Dendritic Cells

作　　者：孙静[1] 李伟伟[1] 华慧[1] 孟一鸣[1] 李璇[1] 单风平[1] 

机构地区：[1]中国医科大学免疫学教研室,辽宁沈阳110001

出　　处：《微生物学杂志》2011年第6期10-14,共5页Journal of Microbiology

基　　金：辽宁省教育厅基金(L2010576)

摘　　要：采用反复冻融法制备Rac-1淋巴瘤细胞总抗原,与树突状细胞(DC2.4)共培养制备DC瘤苗;设置不同实验组,M组及RM组加入蛋氨酸脑啡肽(MENK),研究MENK对DC瘤苗抗肿瘤细胞的杀伤活性影响。结果表明:MENK作用于DC瘤苗后,DC2.4形态上更趋向于成熟,且CD86、MHC-Ⅱ类分子表达水平与对照组相比较明显升高;DC2.4酸性磷酸酶(ACP)含量与对照组相比较明显减少而IL-12的分泌水平升高;同时,DC2.4诱导淋巴细胞增殖能力增强,且诱导活化的淋巴细胞对Rac-1淋巴瘤细胞的杀伤活性与对照组相比较明显增强。结果可见,MENK可明显促进特异性负载Rac-1淋巴瘤抗原的DC2.4的成熟,并增强特异性诱导活化的淋巴细胞对Rac-1淋巴瘤细胞的杀伤活性。General antigen of Rac-1 lymphoma cells were prepared adopting repeated freezing-and-melting process,and co-cultured with dendritic cell（DC2.4） to prepare DC tumour seed;different experiment groups were set up,M group and RM group were added with methionine enkephalin（MENK） to study the effects of killing activity of MENK on antitumor cells of DC tumor seed.The results showed that after MENK working at DC seed,morphologically the DC 2.4 even more tended to mature,and the molecular-like expression level of CD86,MHC-II was significantly fairly higher as compared with control group;the content of DC2.4 acidic phosphatase（ACP） was significantly fairly lower as compared with control group,and the excretion level of IL-12 increased;at the same time,DC2.4 induced the proliferation capacity of lymphocyte to strengthen.And the killing activity of induced activated lymphocyte against Rac-1 lymphoma cell was significantly strengthened as compared with control group.From this experiment it thus concluded that MENK could significantly promote the specific load of Rac-1 lymphoma antigen DC2.4 to mature,and strengthen the specific induced activated lymphocyte killing activity against Rac-1 lymphoma cells.

关 键 词：MENK DC2.4 肿瘤抗原 

分 类 号：R392[医药卫生—免疫学]