桦剥管孔菌液体发酵环境条件的研究  

Optimization of Parameters Affecting Extracellular Polysaccharide Production in Submerged Cultures of Piptoporus betulinus

作　　者：王丽华[1] 田雪梅[1] 毕旺华[1] 梁大勇[1] 王银平[1] 宋爱荣[1] 

机构地区：[1]青岛农业大学药用真菌重点实验室,山东青岛266109

出　　处：《食用菌学报》2011年第4期37-39,共3页Acta Edulis Fungi

基　　金：山东省科技攻关项目(编号:2010GNC10956)的部分研究内容

摘　　要：以发酵液的多糖含量为考核指标,通过单因素试验和正交试验,对桦剥管孔菌(Piptoporus betulinus)液体发酵的接种量、培养基装量及培养基初始pH进行优化。结果表明,优化的桦剥管孔菌培养条件为:接种量2.5%、500mL三角瓶装200mL液体发酵培养基、培养基初始pH自然条件下,发酵6d时,发酵液中多糖含量最高(28.75%)。Orthogonal testing [L9（33）] was used to define more precisely the factors,determined on the basis of single factor experiments,affecting extracellular polysaccharide production in submerged cultures of Piptoporus betulinus.Data revealed that the optimal culture conditions consisted of 2.5% inoculum size,200 mL medium in a 500 mL culture flask,and an initial pH of 5.6.Under these conditions,total polysaccharide concentrations reached 0.18 mg/mL.

关 键 词：桦剥管孔菌 液体培养 培养基装量 接种量 培养基初始pH 

分 类 号：S567.39[农业科学—中草药栽培]