桦褐孔菌不同生长时期胞外酶活性变化  

Extracellular Enzyme Production by Inonotus obliquus During Artificial Solid-state Cultivation

作　　者：赵丽[1] 尹勇刚[1] 傅嫦娥[1] 陈艳秋[1] 

机构地区：[1]长白山生物资源与功能分子教育部重点实验室(延边大学),吉林延吉133002

出　　处：《食用菌学报》2011年第4期55-58,共4页Acta Edulis Fungi

基　　金：国家自然基金资助项目(编号:31160408)的部分研究内容

摘　　要：以桦褐孔菌(Inonotus obliquus)菌株JL01为试验材料,研究其在代料栽培期间9种胞外酶活性的变化规律。结果表明,羧甲基纤维素酶、滤纸纤维素酶和半纤维素酶活性在菌丝生长阶段较低,菌核形成及菌核成熟阶段较高;漆酶、愈创木酚氧化酶、邻苯二酚氧化酶和过氧化物酶活性在菌丝生长阶段高于菌核形成阶段;蛋白酶和淀粉酶活性在菌丝生长时期及菌核成熟时期相对较高。Selected extracellular enzyme activities [carboxymethylcellulase（CMCase）,filter paper enzyme（FPase）,hemicellulase（HCase）,laccase,guaiacol oxidase,catechol oxidase,peroxidase,proteinase and amylase] were determined at different stages during the artificial cultivation of Inonotus obliquus,strain JL01,on a substrate consisting of birch sawdust,wheat bran,corn cobs,sucrose and gypsum.CMCase,FPase and HCase activities were relatively low during the mycelial growth phase but increased between sclerotia initiation and maturation.Laccase,guaiacol oxidase,catechol oxidase and peroxidase activities were relatively high during mycelial colonization of the growth substrate compared with the sclerotia initiation and maturation phases,whereas proteinase and amylase activities were relatively high during the mycelial growth and sclerotia maturation phases.

关 键 词：桦褐孔菌 代料栽培 胞外酶 

分 类 号：S567.39[农业科学—中草药栽培]