桦褐孔菌发酵菌粉成分分析  

Compositional Analysis of Spray-dried Inonotus obliquus Cultures

作　　者：徐欣[1] 杨承建[1] 郑义通[1] 曹佳宁[1] 叶新和[1] 金艳[1] 陆震鸣 史劲松 许正宏 

机构地区：[1]无锡市第二人民医院,江苏无锡214002 [2]江南大学医药学院,江苏无锡214122

出　　处：《食用菌学报》2011年第4期63-66,共4页Acta Edulis Fungi

基　　金：江苏省自然科学基金(编号:BK2010142);国家"863"重点项目子课题(编号:2007AA021506)的部分研究内容

摘　　要：对桦褐孔菌(Inonotus obliquus)发酵菌粉中的各种成分进行了较全面的分析。结果表明发酵菌粉中营养成分较丰富,其中粗蛋白、总氨基酸含量分别为25.73%和15.18%。菌粉中不饱和脂肪酸是优势脂肪酸,以亚油酸和油酸为主,还含有丰富的甾醇、酚类物质和多种矿质元素。用桦褐孔菌发酵菌粉提取粗多糖,得率为28.55%、多糖的分子量主要集中在2～40kDa,单糖组成为阿拉伯糖、甘露糖、葡萄糖、半乳糖,摩尔比为:1.3∶1.0∶20.4∶2.1,多糖产品中含有酸性多糖,糖苷键主要是α型。Inonotus obliquus was grown for 150 h in 1500-L fermenters containing 800 L medium（pH 4.0） consisting of 2% glucose,1% corn powder,1.5% wheat bran extract（15 g wheat bran was suspended in 100 mL deionized water,heated at 90 ℃ for 0.5 h,centrifuged,and 10 mL supernatant diluted 1∶9 with deionized water）,0.3% peptone and 0.1% MgSO4.Compositional analysis of the spray-dried submerged cultures（culture fluid and fungal mycelium） revealed the material to contain crude protein（25.73%）,amino acids（15.18%）,fatty acids（78.02% unsaturated consisting mainly of linoleic and oleic acids）,sterols,phenols and several mineral elements.Water extraction and ethanol precipitation yielded 28.55% polysaccharide（some of which was acidic polysaccharide）,with a molecular weight distribution concentrated mainly within the 2~40 kDa range.The polysaccharide material was composed of arabinose,mannose,glucose and galactose in an overall molar ratio of 1.3∶1.0∶20.4∶2.1 and linked together mainly by α-type glycosidic bonds.

关 键 词：桦褐孔菌 发酵菌粉 化学成分 活性成分 分析 

分 类 号：S567.39[农业科学—中草药栽培]