慢性轻度不可预见性应激对动脉粥样硬化模型大鼠内皮功能的影响  

The influence of chronic unpredictability mild stress on bast function in atherosclerosis model rat

作　　者：赵益[1] 赖小东[1] 苏文[1] 尚广彬[1] 董伟[1] 徐国良[1] 刘红宁[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中药药理与临床》2011年第6期103-105,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江西省科技支撑项目(20111BBG70005-1)

摘　　要：目的:探讨慢性轻度不可预见性应激对高脂诱导动脉粥样硬化模型大鼠内皮功能的影响。方法:雄性大鼠分为空白对照组、应激组、高脂组、应激+高脂组。高脂组给予高脂饲料,应激组给予慢性轻度不可预见性应激。10周后取主动脉弓,做HE染色,并测定血清中血脂、皮质醇(CORT)、一氧化氮(NO)、内皮素-1(ET-1)水平。结果:高脂组与应激+高脂组均可引起大鼠主动脉弓发生AS病变,其中应激+高脂组的病变程度明显强于高脂组;高脂组与应激+高脂组的血清CHO、LDL-C、HDL-C值均高于空白对照组(P<0.05),其中应激+高脂组的CHO、LDL-C、HDL-C值高于高脂组(P<0.01);高脂组与应激+高脂组血清NO和ET-1的含量也明显高于空白对照组(P<0.01),同时应激+高脂组的血清中NO和ET-1的含量高于高脂组(P<0.05);其他三组的皮质醇浓度都比空白对照组低,与其比较都具有统计学意义。结论:慢性应激能够从影响内皮功能上,促进大鼠动脉粥样硬化的发展进程。Objective：To study the influence of chronic unpredictability mild stress on bast function in atherosclerosis model rat which induced by the high-fat diet.Methods：48 healthy wistar male rats were randomly assigned to control group,stress group,high-fat diet group,or stress＋high-fat diet group.The high-fat diet group were feeded with high fat feed,the stress group were given the chronic unpredictability mild stress.After 10 weeks,all rats were sacrificed,took out the aortic arch and prepared for HE staining,serum lipids detection,CORT,ET-1and NO levels detection.Results：The atherosclerosis pathological changes were obviously observed in the high-fat diet group and stress＋high-fat diet group,and the stress＋high-fat diet group was more serious than the high-fat diet group;The serum CHO,LDL-C、HDL-C level in high-fat diet group and stress＋high-fat diet group were higher than the control group（P0.05）,the serum CHO,LDL-C、HDL-C level in stress＋high-fat diet group were obviously higher than high-fat diet group（P0.01）,serum NO level and ET-1 level in high-fat diet group and stress＋high-fat diet group were also clearly higher than Control group（P0.01）,and the latter＇s level were higher than the former＇s level（P0.05）.The CORT level of another three groups were lower than the control group（P0.01,P0.01,P0.05）.Conclusions：The chronic stress can affect the endothelial function to promote the atherosclerosis pathological changes in rats.

关 键 词：慢性轻度不可预见性应激 高脂饲养 内皮功能 动脉粥样硬化 

分 类 号：R285[医药卫生—中药学]