云南传统栽培稻品种waxy基因序列分析  

Sequence Analysis of waxy Gene of Yunnan Rice Landrace

作　　者：李枝桦[1] 陆春明[1] 卢宝荣[2] 王云月[1] 

机构地区：[1]云南农业大学农业生物多样性与病害控制教育部重点实验室,昆明650201 [2]复旦大学生物多样性与生态工程教育部重点实验室,上海200433

出　　处：《分子植物育种》2011年第6期665-671,共7页Molecular Plant Breeding

基　　金：教育部高等学校博士学科点专项基金(200806760001);国家重点基础研究发展计划(2006CB1001205)共同资助

摘　　要：栽培稻是全球最重要的粮食作物之一,waxy基因对栽培稻的品质改良研究具有重要意义。云南是亚洲栽培稻多样性分布中心之一,传统品种丰富。本研究依据云南不同稻区、不同海拔选取99份传统栽培稻品种,对waxy基因序列进行分析。结果显示,供试品种中waxy基因序列包含36个单倍型,其中52个品种存在23bp碱基的插入,而47个品种存在23bp碱基的缺失;糯稻waxy基因第一内含子5'端既有G也有T碱基,剪切位点以T为主,粘稻剪切位点以G为主。7个糯稻地方品种的第一内含子5'端为G,同时第二外显子存在23bp碱基的缺失,与前人的研究结果有所不同,表明云南栽培稻waxy基因单核苷酸多态性高,单倍型多,稻种资源丰富,为挖掘和利用优异糯性基因及品质改良提供了宝贵材料,为云南传统栽培稻种质资源的利用与保护提供了理论依据。Rice （Oryza sativa L.） is the major staple food of the world＇s population. waxy gene is the key gene that determines the starch composition, which is very important of the quality improvement of rice. Yunnan province is one the center of diversity of Asian cultivated rice and many traditional rice varieties were found in Yunnan province. DNA sequence analysis of the 99 Yunnan rice landrace varieties from different attitude and rice planting area. The sequence characterization confirmed that 36 haplotypes of the waxy gene found in the 99 varieties. 52 varieties contain a＂23 bp＂ insertion in the waxy gene, but 47 varieties have a＂23 bp＂ deletion. There are seven glutinous rice varieties that have G/T mutation and the＂23 bp＂ deletion. Yunnan rice varieties had rich SNPs and haploids, which provided valuable rice resource for waxy gene utilization and rice quality improvement. This study provided the scientific basis for conservation of Yunnan rice landrace resources.

关 键 词：WAXY基因 G/T突变 DNA序列多态性 云南地方品种 

分 类 号：S511[农业科学—作物学]