西瓜和甜瓜的SSR引物对三种瓜类作物的通用性分析  

Comparison of Versatility and Polymorphism of Watermelon and Melon SSRs in Three Cucurbitaceae

作　　者：唐鑫[1] 张海英[2] 许勇[2] 程蛟文[1] 杨衍[3] 胡开林[1] 

机构地区：[1]华南农业大学园艺学院,广州510640 [2]北京市农林科学院蔬菜研究中心,北京100097 [3]中国热带农业科学院热带作物品种资源研究所,儋州571737

出　　处：《分子植物育种》2011年第6期760-764,共5页Molecular Plant Breeding

基　　金：广东现代农业产业技术体系建设专项资金资助

摘　　要：利用西瓜的1824对SSR引物和甜瓜的259对SSR引物,对丝瓜、冬瓜和苦瓜的DNA进行了PCR扩增,分析了西瓜和甜瓜SSR引物对三种瓜类作物扩增的通用性以及三种瓜类作物中不同类型之间的多态性。结果表明,西瓜和甜瓜SSR引物对丝瓜、冬瓜和苦瓜的DNA扩增通用性分别是15.31%、23.04%和14.74%。但是,在三种瓜类作物不同类型之间具有多态性扩增的SSR引物较少,其中在有棱丝瓜和无棱丝瓜之间、冬瓜和节瓜之间、8个不同特性苦瓜材料之间具有多态性扩增的SSR引物分别只占所有筛选引物的3.46%、0.38%和1.68%。In this study, by PCR amplification with the genomic DNA of towel gourd, wax gourd and bitter gourd, 1 824 watermelon SSR primers and 259 melon SSR primers were tested for analyzing their versatility and polymorphism of various types among three cucurbitaceae crops. The results indicated that the versatility of SSR primers amplification in towel gourd, wax gourd and bitter gourd was 15.31%, 23.04% and 14.74%, respectively. However, the polymorphism level of all these SSR primers among three cucurbitaceae crops and various types was very low. The ratio of polymorphism SSR primers between ridge gourd and sponge gourd, wax gourd and chieh-qua and among eight bitter gourds from different origins was 3.46%, 0.38% and 1.68%, respectively.

关 键 词：SSR 丝瓜 冬瓜 苦瓜 通用性 多态性 

分 类 号：S642[农业科学—蔬菜学]