牵牛子杀螨活性物质鉴定及其作用机制初探  

A Preliminary Study on Activity Mechanism and Identification of Extract of Pharbitis purpurea Seeds against Tetranychus cinnabarinus

作　　者：杜娟[1] 赵磊[1] 师光禄[1] 王有年[1] 仝宝生 

机构地区：[1]农业部都市农业(北方)重点开放实验室北京农学院生物技术学院,北京102206 [2]内蒙古永业生物技术有限责任公司,呼和浩特010010

出　　处：《林业科学》2011年第11期199-203,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金资助项目(30872029);北京市自然科学基金重点项目(6071001);北京市自然科学基金项目(6092007);北京市教委重点项目(KZ201010020016);北京市教委科研基地-科技创新平台建设项目(PXM2011_014207_000032);北京市教委科技成果转化与产业化(PXM2011_014207_000026);北京市属高校人才强教深化计划项目(PHR20090516;PHR200906134)

摘　　要：朱砂叶螨（Tetranychus cinnabarinus）具有个体小、繁殖快、代数多、适应性强、为害重等特点,是世界公认的最难防治的有害生物群落（Hazan et al.,1974;何林等,2004）,可危害百余种农作物和果树,并对其产量和品质造成严重的影响（Ho et al.,1997）。长期以来,大量重复使用非专一性化学杀螨剂,使许多螨类已产生抗药性（唐除痴等,1998）,并且对杀螨剂出现了交互抗性（王旭等,1997）。Fraction 8 of the extractants from semen pharbitidis was identified and its mechanism of biological activity against Tetranychus cinnabarinus was investigated. The main component of the extractant fraction 8 was detected with nuclear magnetic resonance （NMR）, gas chromatography and mass spectrum （GC-MS）. Influences of the main component on several enzymes activities such as glutathione-S-transferase （GSTs）, acetylcholine esterase （AChE） and mono-amine oxidase （MAO）, in T. cinnabarinus body were detected by using biochemical method. Results showed that the main component of the extractant fraction 8 was methyl linoleate. The methyl linoleate activated glutathione-S-transferase in T. cinnabarinus body, while it inhibited to various degrees acetylcholine esterase and mono-amine oxidase related to nervous system, and hence caused the death of T. cinnabarinus. Therefore the methyl linoleate has a good prospect developing into new pesticide.

关 键 词：朱砂叶螨 亚油酸甲酯 谷胱甘肽-S-转移酶 乙酰胆碱酯酶 单胺氧化酶 CA2+-ATP酶 Na+ K+-ATP酶 

分 类 号：S436.619[农业科学—农业昆虫与害虫防治]