草兔线粒体基因组全序列及其系统发育关系分析(英文)  

Complete mitogenome of cape hare Lepus capensis(Lagomorpha:Lepo-ridae)and its phylogenetic considerations

作　　者：王加连[1,2] 杨光[1] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,南京210046 [2]盐城师范学院,江苏省滩涂生物资源与环境保护重点建设实验室,盐城224002

出　　处：《兽类学报》2012年第1期1-11,共11页Acta Theriologica Sinica

基　　金：financially supported by the Program for New Century Excellent Talents in University(NCET-07-0445);the Ministry of Education of China,and the major project of the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(07KJA18016)

摘　　要：草兔(Lepuscapensis)在我国数量多,分布广,但人们对草兔的系统地理学、草兔与其他兔类的系统发育关系了解不多,其亚种水平的分类也长期存在争议。本研究通过LA-PCR技术对草兔线粒体基因组全序列进行PCR扩增、序列测定和分析,并基于线粒体基因组12个蛋白质编码基因10776bp的核苷酸序列,采用贝叶斯法(BI)和最大似然法(ML)构建哺乳纲(Mammalia)灵长总目(Euarchontoglires)5个目12种动物的系统发育关系,结果支持兔形目(Lagomorpha)的单系起源,其中草兔与欧洲野兔(L.europaeus)亲缘关系最近,两者互为姐妹群,进而与家兔(Oryctolagus cuniculus)构成姐妹群关系。此外,基于线粒体细胞色素b基因全序列,构建31个兔类个体的系统发育关系,结果显示,草兔与雪兔(L.timidus),云南兔(L.comus)与高原兔(L.oiostolus),东北兔(L.mandschuricus)与塔里木兔(L.yarkandensis)有较近的亲缘关系,而在草兔内部,中国的草兔与南非草兔亲缘关系较远,采自江苏盐城的草兔与来自山东、陕西、四川的草兔聚为一支,具有明显较近的亲缘关系。该研究为更好地探讨草兔系统发育关系提供了进一步的分子生物学资料。Despite its wide distribution and abundant wild populations,there has been little insight into the phylogeography of the Chinese cape hare（Lepus capensis） and its relationships with other hare species.Moreover,its subspecies taxonomic status has been under debate for years.In present study,we reporte the complete mitochondrial genomic sequence of the cape hare from Yancheng City,Jiangsu Province,China,and that the gene composition /order of which were identical to that observed in most other mammals.Phylogenetic relationships of 12 species from five orders of Euarchontoglires,based on mitogenomic sequences,were reconstructed using Bayesian inference（BI） and maximum likelihood（ML） methods.The results strongly supported the monophyly of Lagomorpha,and cape hare and brown hare（L.europaeus） were indicated as sister taxa,having a sister relationship with rabbit（Oryctolagus cuniculus）.Further phylogenetic analyses of 31 hare samples,based on complete Cyt b sequences,resolved the close phylogenetic relationships between cape hare and mountain hare（L.timidus）,Yunnan hare（L.comus） and woolly hare（L.oiostolus）,Manchurian hare（L.mandschuricus） and Yarkand hare（L.yarkandensis）,respectively.The cape hare collected from Yancheng,Jiangsu in our study,had a close phylogenetic relationship with those from Shandong,Shaanxi and Sichuan Provinces.The present results provided some new insights into the tangled phylogenetic relationships of the cape hare.

关 键 词：线粒体基因组 系统发育分析 草兔 

分 类 号：Q349[生物学—遗传学]