宫颈病变患者人乳头状瘤病毒感染状况及其亚型分布的研究  

Human papilloma virusinfection in patients with cervical lesions and distribution of its subtypes

作　　者：方美玉[1] 屠巧凤[1] 沈宝珠[2] 

机构地区：[1]杭州市第一人民医院检验科,浙江杭州310006 [2]杭州市第一人民医院病理科,浙江杭州310006

出　　处：《中华医院感染学杂志》2012年第4期685-687,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨宫颈病变患者人乳头状瘤病毒(HPV)的感染状态及其亚型分布,提出预防治疗疾病的干预措施。方法采用导流杂交基因芯片技术检测600例宫颈病变患者宫颈脱落细胞HPV亚型,分析不同宫颈病变患者的HPV感染情况。结果 HPV感染率为78.0%,其中宫颈炎/尖锐湿疣高危型及低危型HPV感染率为73.1%、18.1%,差异有统计学意义(P<0.05);CIN患者高危型及低危型HPV感染率为82.6%、7.9%,差异有统计学意义(P<0.01);宫颈癌患者高危型及低危型HPV感染率为96.0%、0,差异有统计学意义(P<0.01);宫颈炎/宫颈尖锐湿疣、CIN和宫颈癌高危型HPV感染率明显高于低危型(P<0.01);高危型HPV感染率随宫颈病变程度加重而升高;各种宫颈病变中单一型感染率明显高于多重型感染率(P<0.01);多重型感染率随宫颈病变程度加重而升高;不同HPV亚型在各病变中分布不同。结论 HPV亚型检测可用于宫颈病变的筛查及病情判断,对HPV阳性特别是高危型HPV感染的人群关键为早期诊断,及时治疗,跟踪监测,以阻断HPV的持续性感染,预防宫颈癌的发生。OBJECTIVE To investigate human papilloma virus（HPV） infections and the distribution of its subtypes in patients with cervical lesions and put forward counter measures for prevention and treatment of the disease.METHODS Cervical exfoliated cells collected from a total of 600 patients with cervical lesions were detected with Flow-through hybridization and gene chip technology,and followed by a complete evaluation of the HPV infection in related to different subtypes of cervical lesions.RESULTS The total HPV infection rate reached 78.0%.The high-risk and low-risk HPV infection rates of patients with cervicitis/ condyloma acuminatum were 73.1% and 18.1%,respectively.The difference was statistically significant（P〈0.05）.The high-risk and low-risk HPV infection rates of patients with cervical intraepithelial neoplasia（CIN） were 82.6% and 7.9%,respectively,the difference was statistically significant（P〈0.01）.The high-risk and low-risk HPV infection rates of patients with cervical cancer were 96.0% and 0,respectively,the difference was statistically significant（P〈0.01）.The high-risk HPV infection rate increased with the degree of exacerbating of cervical lesions.The rate of single-type HPV infection was significantly higher than that of multi-type HPV infection in all kinds of cervical lesions（P〈0.01）,while the rate of multi-type HPV infection increased with the increasing severity of cervical lesions.Different subtypes of HPV distributed differently in cervix lesions.CONCLUSION Detection of HPV subtypes is helpful for screening of the cervical lesions and diagnosis of the condition.For those patients infected especially with high-risk HPV,it is critical to conduct the early diagnosis,timely treatment and close follow-up to block the persistent HPV infection and prevent cervical cancer.

关 键 词：宫颈病变 人乳头状瘤病毒 感染 导流杂交基因芯片技术 

分 类 号：R373[医药卫生—病原生物学]