新型生物素接枝聚乳酸纳米球的制备及性能研究  

The preparation and property of nanoparticles of a novel biotin-graft-poly(lactic acid) material

作　　者：江伟民[1,2] 潘君[1,2] 严好[1,2] 罗春花[1,2] 张寅星[1,2] 龚啸元[1,2] 王素军[1,2] 王远亮[1,2] 

机构地区：[1]重庆大学生物工程学院,生物材料与仿生工程中心,重庆400044 [2]重庆大学生物流变科学与技术教育部重点实验室,重庆400044

出　　处：《功能材料》2012年第4期417-420,共4页Journal of Functional Materials

基　　金：中央高校基本科研业务费资助项目(CDJZR11230004)

摘　　要：利用自组装方式制备生物素接枝聚乳酸材料(BPLA)的纳米球,对其性能进行表征;体外探讨BPLA纳米球与链霉亲和素和生物素的结合能力,据此判断BPLA纳米球的潜在靶向性。结果表明BPLA纳米球呈球形,平均粒径<200nm,分散系数<0.15,平均电位<-25mV;静置1月稳定;浓度为0.01～1.0mg/mL的BPLA纳米球的溶血率均低于5%;在37℃体外模拟体液静止和流动情况下能够与链霉亲和素进行结合;同时,在以链霉亲和素为臂时,BPLA纳米球仍然具备与生物素特异性结合能力。显示出BPLA纳米球在药物靶向领域具有潜在应用前景。Nanoparticles（NPs） were prepared by self-assembly method using our newly synthesized biotin-graft-poly（lactic acid）（BPLA）.The NPs were characterized in terms of morphology,particle size distribution,zeta potential and hemolytic activity.The specific combination of the BPLA NPs with streptavidin or biotin via streptavidin arm in vitro were tested in static and dynamic condition,from which the potential targeted property of the BPLA NPs could be judged.The results show that the BPLA NPs were spherical with particle size,zeta potential and PDI less than 200 nm,-25mV and 0.15.It stayed stable for over one month.The hemolytic rate of BPLA NPs with concentrations in the range of 0.01-1.0 mg/mL was less than 5%.The BPLA NPs combined with streptavidin and biotin using streptavidin as an arm on the simulated in vivo static and dynamic flow condition.The results suggest BPLA NPs might have a potential application in target drug delivery.

关 键 词：聚乳酸 生物素 链霉亲和素 纳米球 靶向 

分 类 号：R318.08[医药卫生—生物医学工程]