血红蛋白在戊二醛膜修饰金电极上的直接电化学行为及其与氨茶碱的相互作用  

Direct electrochemical behavior of hemoglobin in glutaraldehyde film modified gold electrode and its interaction with aminophyline

作　　者：闵文傲[1] 王卫平[1] 袁军华[1] 陈建荣[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2012年第1期75-79,共5页Journal of Zhejiang Normal University:Natural Sciences

基　　金：浙江省教育厅项目(Y200805462);浙江师范大学博士科研启动基金资助项目(ZC304007104)

摘　　要：用直接滴涂法将血红蛋白(Hb)固定到戊二醛(GA)膜修饰的金电极表面,通过循环伏安法研究了Hb在GA膜修饰金电极上的直接电化学行为.在0.1 mol/L磷酸盐缓冲溶液(PBS,pH 6.00)中,扫描速度为100mV/s时,该修饰电极的峰电位差ΔE=44 mV,氧化还原峰电流之比Ip,a/Ip,c=1.17,说明Hb在GA膜修饰电极上的氧化还原过程是可逆过程;随着缓冲溶液pH值的增大,其峰电位不变,而峰电流呈增大(pH=4.00～6.00)或减小(pH=6.00～8.00)的趋势.通过GA膜对Hb的吸附固定,不仅保持了Hb的生物活性,而且实现了Hb与电极之间的直接电子转移.利用此修饰电极,研究了Hb与药物氨茶碱之间的相互作用,求得Hb和氨茶碱的结合数为2.A steady immobilized-hemoglobin modified electrode was obtained successfully by immobilizing hemoglobin on a gold electrode, which was modified with glutaraldehyde films. Direct electrochemical behavior of hemoglobin on glutaraldehyde films was investigated by Cyclic Voltammetry. Under the conditions of PBS （ pH 6.00 ） and scan rate 100 mV/s, peak to peak separation and peaks current ratio were 44 mV and 1.17, respectively, which indicated that the redox process of hemoglobin on the modified electrode was reversible. Peak potential remained unchanged with pH increased in the range of 4.00 - 8.00, while peak current showed increasing （ pH = 4.00 - 6.00 ） or decreasing （ pH = 6.00 - 8.00 ） trend. The results showed that with glutaraldehyde to immobilize hemoglobin, hemoglobin not only kept its biological activity, but also realized the direct electron transfer mechanism between hemoglobin and gold electrode. The interaction between hemoglobin and aminophylline was also investigated by using the modified electrode.

关 键 词：血红蛋白 戊二醛 循环伏安法 修饰电极 氨茶碱 

分 类 号：O657.8[理学—分析化学]