Combined postconditioning with ischemia and α7nAChR agonist produces an enhanced protection against rat myocardial ischemia reperfusion injury  

作　　者：XIONG Jun YUAN Yu-jing XUE Fu-shan WANG Qiang LI Shan LIAO Xu LIU Jian-hua CHEN Yi LI Rui-ping 

机构地区：[1]Department of Anesthesiology, Tongren Hospital, Capital Medical University, Beijing 100730, China [2]Department of Anesthesiology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100144, China

出　　处：《Chinese Medical Journal》2012年第2期326-331,共6页中华医学杂志（英文版）

摘　　要：Background Inflammation is one of important mechanisms for myocardial ischemia reperfusion injury （IRI）.Ischemia postconditioning （IPOC） can protect the heart against IRI by inhibiting inflammation,but its cardioprotection is weaker than that of ischemia preconditioning.Recently,the α7 subunit-containing nicotinic acetylcholine receptor （α7nAChR） agonist has shown anti-infiammatory effects in many diseases related to inflammation.This randomized controlled experiment was designed to evaluate whether combined postconditioning with IPOC and the α7nAChR agonist could produce an enhanced cardioprotection in a rat in vivo model of acute myocardial IRI.Methods Fifty Sprague-Dawley rats were randomly divided into five equal groups：sham group,control group,IPOC group,α7nAChR agonist postconditioning group （APOC group） and combined postconditioning with IPOC and α7nAChR agonist group （combined group）.Hemodynamic parameters were recorded during the periods of ischemia and reperfusion.Serum concentrations of troponin I （Tnl）,tumor necrosis factor α （TNF-α） and high-mobility group box 1 （HMGB-1） at 180 minutes after reperfusion were assayed in all groups.At the end of the experiment,the infarct size was assessed from excised hearts by Evans blue and triphenyl tetrazolium chloride staining.Results As compared to the sham group,the infarct size in the other four groups was significantly increased,serum levels of Tnl,TNF-α and HMGB1 in the control group and TNF-α,HMGB1 in the IPOC group were significantly increased.The infarct size and serum concentrations of TNF-α,HMGB1 and Tnl in the IPOC,APOC and combined groups were significantly lower than those in the control group.As compared to the IPOC group,the infarct size in the combined group was significantly decreased,serum concentrations of Tnl,TNF-α and HMGB1 in the APOC and combined groups were significantly reduced.Although the infarct size was significantly smaller in the combined group than in the APOC group,serum levels of TNF-�Background Inflammation is one of important mechanisms for myocardial ischemia reperfusion injury （IRI）.Ischemia postconditioning （IPOC） can protect the heart against IRI by inhibiting inflammation,but its cardioprotection is weaker than that of ischemia preconditioning.Recently,the α7 subunit-containing nicotinic acetylcholine receptor （α7nAChR） agonist has shown anti-infiammatory effects in many diseases related to inflammation.This randomized controlled experiment was designed to evaluate whether combined postconditioning with IPOC and the α7nAChR agonist could produce an enhanced cardioprotection in a rat in vivo model of acute myocardial IRI.Methods Fifty Sprague-Dawley rats were randomly divided into five equal groups：sham group,control group,IPOC group,α7nAChR agonist postconditioning group （APOC group） and combined postconditioning with IPOC and α7nAChR agonist group （combined group）.Hemodynamic parameters were recorded during the periods of ischemia and reperfusion.Serum concentrations of troponin I （Tnl）,tumor necrosis factor α （TNF-α） and high-mobility group box 1 （HMGB-1） at 180 minutes after reperfusion were assayed in all groups.At the end of the experiment,the infarct size was assessed from excised hearts by Evans blue and triphenyl tetrazolium chloride staining.Results As compared to the sham group,the infarct size in the other four groups was significantly increased,serum levels of Tnl,TNF-α and HMGB1 in the control group and TNF-α,HMGB1 in the IPOC group were significantly increased.The infarct size and serum concentrations of TNF-α,HMGB1 and Tnl in the IPOC,APOC and combined groups were significantly lower than those in the control group.As compared to the IPOC group,the infarct size in the combined group was significantly decreased,serum concentrations of Tnl,TNF-α and HMGB1 in the APOC and combined groups were significantly reduced.Although the infarct size was significantly smaller in the combined group than in the APOC group,serum levels of TNF-�

关 键 词：ischemia reperfusion injury ischemia postconditioning pro-informatory cytokines α7 subunit-containing nicotinic acetylcholine receptor pharmacologicalpostconditioning 

分 类 号：R541[医药卫生—心血管疾病]