天花粉提取物对HepG2.2.15细胞HBsAg和HBeAg表达的影响  

Effect of extracts from Radix Trichosanthis on the expression of HBsAg and HBeAg in HepG2.2.15 cells

作　　者：陈佳[1] 许昭发[1] 欧阳红涛[2] 彭敏源[3] 吴婷[1] 刘静[1] 刘艳平[1] 言惠文[1] 

机构地区：[1]中南大学生物科学与技术学院,长沙410013 [2]湖南省第二人民医院,长沙410007 [3]中南大学湘雅医院血液科,长沙410008

出　　处：《中南大学学报（医学版）》2012年第1期38-41,共4页Journal of Central South University ：Medical Science

基　　金：国家自然科学科学基金(30971517);中南大学"大学生创新课题"(YD09194)~~

摘　　要：目的:探讨天花粉水提取物、醇提取物对HepG2.2.15细胞的增殖及培养细胞上清中HBsAg与HBeAg表达的影响。方法:用MTT方法检测HepG2.2.15细胞的存活率,以酶联免疫法(ELISA)检测HepG2.2.15细胞上清液HBsAg和HBeAg的表达量。结果:天花粉水提取物和醇提取物处理HepG2.2.15细胞后HBsAg和HBeAg的表达均呈时间依赖性和浓度依赖性抑制,但天花粉水提物的治疗指数明显优于醇提物。结论:天花粉水提物较醇提物有更好的抗HBV活性。Objective： To investigate the effect of extracts with water and alcohol from Radix Trichosanthis on the cell survival and the expression of hepatitis B surface antigen（HBsAg） and hepatitis B e-antigen（HBeAg） in HepG2.2.15 cell supernatant. Methods： The cell survival rate of HepG2.2.15 cells was detected by MTT assay.The HBsAg and HBeAg in HepG 2.2.15 cell supernatant were evaluated by enzyme linked immunosorbent assay. Results： The water and alcohol soluble extracts from Radix Trichosanthis significantly inhibited the levels of HBsAg and HBeAg in HepG2.2.15 cells in a time-and-concentration-dependent manner.However,the therapeutic index of extracts with water from Radix Trichosanthis was better than that in the alcohol group. Conclusion： The activity of water-soluble extract from Radix Trichosanthis is stronger on anti-hepatitis B virus than that of the alcohol-soluble extract.

关 键 词：天花粉 HEPG2.2.15细胞 HBSAG HBEAG 

分 类 号：R285[医药卫生—中药学]