大鼠脑微透析样品中左旋多巴、多巴胺及其代谢产物、羟自由基的测定  

Simultaneous determination of L-DOPA,DA and its metabolites,hydroxyl free radical in brain microdialysis of rats

作　　者：吴兆恩[1] 牛晓红[1] 张美玉[1] 王志国[1] 李鹏[1] 王丹巧[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700

出　　处：《药物分析杂志》2012年第2期217-220,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金资助项目(30772780);国际科技合作资助项目(2010DFA31890)

摘　　要：目的:建立快速、准确、同时测定大鼠脑微透析样品中左旋多巴(L-DOPA)、多巴胺(DA)及其代谢产物、羟自由基指标的高效液相色谱-电化学检测方法。方法:应用玻璃碳工作电极、银/氯化银(Ag/AgCl)参比电极、Antec Leyden BV C18分析柱,工作电压为0.52 V,单一流动相,检测大鼠脑微透析样品。结果:清醒自由活动大鼠纹状体微透析液中L-DOPA、DA及其代谢产物、羟自由基指标等10种物质在15 min内分离。标准曲线在一定浓度内线性良好。结论:所建方法可用于大鼠脑微透析样品中L-DOPA、DA及其代谢产物等的同时测定,为脑内L-DOPA药物浓度-药物效应-药物副作用同步研究提供可用的方法。Objective： To establish a rapid, accurate HPLC - ECD method to simultaneously detect the L - dihydroxyphenylalanine （ L - DOPA ）, dopamine （ DA ） and its metabolites, hydroxyl free radical. Method： The aria- lyres were separated on a Antec Leyden BV C18 column（3 μm,2. 1 mm× 100 mm） under the same mobile phase. The electrochemical detector was equipped with a glass carbon working electrode and a Ag/AgC1 reference electrode. The working voltage was 0. 52 V. Result： The extracellular L - dihydroxyphenylalanine （ L - DOPA）, DA and its metabolites,hydroxyl free radical in striatum of free -moving rats can be separated in 15 rain. The calibration curves were linear within the limited concentration ranges. Conclusion：This method can be used to determine simultaneously the concentrations of L - DOPA, DA and its metabolites from the brain microdialysis of rats, providing the possible method of simultaneously measuring the concentration of L - DOPA in brain, target efficacy and studying the side effects of drugs.

关 键 词：生物样品 左旋多巴(L-DOPA) 多巴胺(DA) 代谢产物 羟自由基 高效液相色谱 电化学检测 微透析技术 

分 类 号：R917[医药卫生—药物分析学]