HPLC同时测定山核桃外蒲壳中球松素和大黄酚含量  

HPLC simultaneous determination of pinostrobin and chrysophanol in hull of Carya cathayensis Sarg

作　　者：吴德玲[1,2] 陈仕云[1,3] 金传山[1,2] 刘劲松[1,2] 彭代银[2,4] 

机构地区：[1]安徽中医学院药学院,合肥230031 [2]安徽省现代中药重点实验室,合肥230031 [3]合肥学院化学与材料工程系,合肥230031 [4]现代中药安徽省工程技术研究中心,合肥230031

出　　处：《药物分析杂志》2012年第2期249-251,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：安徽中医学院合作研究项目(2008HZ001)

摘　　要：目的:建立同时测定山核桃外蒲壳中球松素和大黄酚含量的分析方法。方法:采用HPLC法,色谱柱为Shim-pack C18(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸水溶液(79∶21)为流动相,流速0.8 mL·min-1,检测波长286 nm,柱温25℃。结果:山核桃外蒲壳中球松素和大黄酚的线性范围分别为4.12～25.7μg·mL-1和1.01～13.4μg·mL-1,平均加样回收率(n=6)分别为98.8%和98.0%。结论:该方法准确可靠、结果稳定、重复性好,为评价山核桃外蒲壳的质量提供依据。Objective：To establish a method for simultaneous determination of pinostrobin and chrysophanol in the hull of Carya cathayensis. Methods：The analysis was carried out on a Shim -pack C18 （250 mm ×4.6 mm,5 μm） column eluted with a mobile phase of methanol -water containing 0. 1% phosphoric acid （79：21 ） ;The flow rate was 0. 8 mL ~ min-1 and column temperature was 25 ℃ ;The detection wavelength was set at 286 nm. Results：The calibration curves of pinostrobin and chrysophanol were linear in the ranges of 4. 12 -25.7 μg ·mL-1 and 1.01 - 13.4 μg ·mL-1, and the average recoveries （n = 6 ） were 97.8 % and 98.0%, respectively. Conclusion：The method is simple, accurate and sensitive, which can be used as a method of quantitative determination of pinostrobin and chrysophanol in the hull of Carya cathayensis.

关 键 词：山核桃外蒲壳 球松素(乔松酮) 大黄酚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]