CD4+CD25+调节性T细胞及TGF-β1与系统性红斑狼疮病情活动性关系的研究  

Study on relationship between CD4+CD25+ regulative T cells,TGF-β1 and activity of systemic lupus erythematosus

作　　者：詹淑霞[1] 陈路 陆东庆[3] 

机构地区：[1]深圳市蛇口人民医院皮肤科,广东深圳518067 [2]深圳市南山人民医院肾内科,广东深圳518052 [3]中山大学附属第五医院皮肤性科,广东珠海519000

出　　处：《临床和实验医学杂志》2012年第4期250-251,253,共3页Journal of Clinical and Experimental Medicine

基　　金：深圳市南山区科技局资助项目;项目编号[南卫2009027]

摘　　要：目的检测系统性红斑狼疮患者(SLE)外周血CD4+CD25+调节性T细胞、转化生长因子-β1(TGF-β1)浓度,探讨其与疾病活动性的关系。方法选择SLE患者76例,根据患者病情程度分为3组,其中稳定组17例,活动组33例,肾病组26例。同期选择24例健康体检者作为正常对照。采用流式细胞仪检测SLE患者外周血CD4+CD25+调节性T细胞群比率,用ELISA法检测血清中TGF-β1浓度。观察CD4+CD25+T细胞群、TGF-β1浓度与SLE患者疾病活动程度的关系。结果①活动期SLE患者外周血CD4+CD25+调节性T细胞群比率、TGF-β1浓度显著低于稳定期及正常对照组(P<0.01),疾病稳定期与正常对照组结果差异无统计学意义。②SLE并发肾病组外周血CD4+CD25+调节性T细胞群比例、TGF-β1浓度显著低于非肾病组(P<0.05)。结论 SLE患者外周血CD4+CD25+调节性T细胞群比率、TGF-β1浓度与SLE活动性关系密切,可能是导致疾病发生和病情发展的关键环节之一。Objective To study the percentage of peripheral blood CD4＋CD25＋ regulative T cells and serum level of TGF-β1 in patients with systemic lupus erythematosus（SLE） and to explore their relationship with activity of the disease.Methods The percentage of CD4 ＋CD25＋ T cells in peripheral blood from patients with systemic lupus erythematosus（SLE） and normal controls was measured by flowcytometry.Serum level of TGF-β1 was detected by double antibody sandwich ELISA.Results Patients with active disease had statisitically lower levels of CD4＋CD25＋ T cells and TGF-β1 than those of normal controls and patients with non-active disease（P〈0.01）,whereas no significant difference could be found between patients with non-active disease and normal controls.SLE patients with nephropathy had significantly lower levels of CD4＋CD25＋T cells and TGF-β1 than those of patients without nephropathy（P〈0101）.Conclusion The decrease of percentage of peripheral blood CD4＋CD25＋T cells and serum level of TGF-β1 in patients with SLE is closely related with the activity of disease and complicated nephropathy.The decrease in percentage of blood CD4＋CD25＋T cells and serum level of TGF-β1 may play a crucial role in pathogenesis and progress of SLE.

关 键 词：系统性红斑狼疮 CD4+CD25+T细胞 TGF-Β1 外周血 

分 类 号：R593.241[医药卫生—内科学]