广东神曲显微鉴别及含量测定(HPLC法)的研究  

The research of microscopical identification and content determination (HPLC method) for Guangdong Shenqu

作　　者：周燕双 黄惠莲 宋丽莉 

机构地区：[1]广州王老吉药业股份有限公司,广东广州510450

出　　处：《中国当代医药》2012年第2期43-45,共3页China Modern Medicine

摘　　要：目的:研究提高广东神曲的质量标准,建立具有特征性的广东神曲显微鉴别和高效液相含量测定。方法:采用中药材粉末显微鉴定,寻找广东神曲在显微镜下的茯苓和猪苓药材的特征性细胞;用高效液相法测定广东神曲中黄芩药材的含量。结果:显微鉴定显示茯苓和猪苓药材特征性强;含量测定方法显示黄芩药材中黄芩苷检测操作简单,准确,黄芩苷在10.25～328.00μg之间呈良好的线性关系,黄芩苷的回收率为99.82%。结论:对茯苓和猪苓药材定性及黄芩药材定量方法准确、简便,可用于控制广东神曲的质量。Objective： To improve the quality standard of Guangdong Shenqu, methods of microscopical identification for the characteristic identification and a HPLC method for determining the content of Guangdong Shenqu were established. Methods： Microscopical identification method for identification of the herbal powder was used to look for the characteristic cells of the Poria cocos and the Agaric; HPLC was used to determine the content of Baicalin in the Scutellaria Baicalensis. Results： Microscopical identification showed that The Poria cocos and the Agaric had strong characters; HPLC result showed that this method was easy to handle and accurate. In the range of 10.25-328.00 μg, the baicalin showed good linear relationship. The recovery of baicalin was 99.82%. Conclusion： The microscopical identification method for identification of the Poria cocos and the Agaric and the HPLC method for determining the content of Baicalin are both accurate and easy, can be used to control the quality of Guangdong Shenqu.

关 键 词：广东神曲 茯苓和猪苓药材 黄苓苷 显微鉴别 高技液相色谱法 

分 类 号：R284[医药卫生—中药学]