毛细管电泳电化学法分离检测盐酸克伦特罗、特布他林和沙丁胺醇  

Separation and determination of clenbuterol hydrochloride,terbutaline and salbutamol by capillary electrophoresis with electrochemical detection

作　　者：邓光辉[1] 陈盛余[1,2] 高静[1] 王辉[1] 

机构地区：[1]广西民族大学化学与生态工程学院,化学与生物转化过程新技术广西高校重点实验室,南宁530006 [2]广西化工研究院,南宁530001

出　　处：《分析试验室》2012年第2期25-28,共4页Chinese Journal of Analysis Laboratory

基　　金：广西自然科学基金项目(桂科能0640038);广西科学研究与技术开发计划项目(桂科能0992028-13)资助

摘　　要：建立了毛细管电泳电化学法对盐酸克伦特罗、特布他林和沙丁胺醇进行分离检测。方法采用胶束电泳体系,以铂圆盘为工作电极,考察了检测电位、缓冲液浓度和pH、十二烷基硫酸钠(SDS)浓度、分离电压等因素的影响。3个分离物在10 kV的分离电压、缓冲体系为15 mmol/L(pH 9.0)硼砂+20 mmol/L SDS条件下得到分离。盐酸克伦特罗、特布他林和沙丁胺醇的线性范围分别为2.0～400,3.5～700,5.0～1000μg/L。方法已用于猪肉样品的检测。Micellar electrokinetic capillary chromatography（MECC） with electrochemical detection has been developed for the separation and determination of clenbuterol hydrochloride,terbutaline and salbutamol in pork.The detection electrode was a platinum disc electrode at a detection potential of ＋1.00V（versus Ag/AgCl electrode）.The effects of the acidity and the concentration of the running buffer,separation voltage,injection time,and detection potential were investigated to acquire the optimum conditions.The analytes were baseline separated within 17 min at a separation voltage of 10 KV in a 15 mmol/L sodium borate buffer（pH9.00） containing 20 mmol/L sodium dodecyl sulfate（SDS）.Linear ranges for clenbuterol hydrochloride,terbutaline and salbutamol were 2.0～400 μg/L,3.5～700 μg/L,and 5.0～1000 μg/L,respectively.

关 键 词：毛细管电泳 电化学检测 盐酸克伦特罗 特布他林 沙丁胺醇 

分 类 号：O657.8[理学—分析化学]