少数民族人群hOGG1基因多态性与生活方式及DNA氧化损伤的关系  

Association of hOGG1 genotype with life style and oxidative DNA damage in five ethnic populations in China

作　　者：吴双[1] 蒋友胜[1] 袁建辉[1] 庄志雄[1] 柯跃斌[1] 

机构地区：[1]深圳市疾病预防控制中心

出　　处：《卫生研究》2012年第1期45-50,共6页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30872149/C1504);广东省自然科学基金项目(No.8451802003000336)

摘　　要：目的为评估hOGG1基因自然变异和基因-环境的交互作用,对中国5个民族人群的953个健康个体的hOGG1基因326位多态性分布进行了调查。方法用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析hOGG1基因326位密码子多态性,用高效液相色谱-电化学检测（HPLC-ECD）方法分析尿中8-羟基-脱氧鸟嘌呤（8-OHdG）水平,生活方式相关因素（吸烟、饮酒、肉类和水果摄入）资料通过问卷调查获得。结果中国人群中hOGG1基因的等位基因频率分别为0.16（Ser/Ser）,0.49（Ser/Cys）和0.35（Cys/Cys）。5个民族人群中Ser326Cys多态性频率具有显著性差异（P=0.002）。除Ser326Cys与吸烟有关联外（P=0.027）,hOGG1基因多态性与其他的生活方式因素没有关系。Cys326Cys等位基因健康受试者尿中8-OHdG水平显著升高（P=0.033）。结论各民族人群hOGG1基因存在自然变异。吸烟与Cys/Cys多态性频率有关,hOGG1 Cys326Cys基因型个体DNA氧化性损伤的修复水平较低。Objective To assess the natural variation of hOGG1 gene and the gene-environmental interactions.Methods The hOGG1 codon 326 polymorphism and urinary 8-OHdG levels were investigated in a large sample of healthy individuals（n=953） from five ethnic populations in China by using PCR-RFLP and HPLC-ECD.Life style parameters were obtained through a questionnaire survey.Results The allelic frequencies of hOGG1 gene in whole subjects were 0.16（Ser/Ser）,0.49（Ser/Cys） and 0.35（Cys/Cys）.The frequency of Ser326Cys was significantly different among the five ethnic populations（P=0.002）.No association was found between the hOGG1 gene polymorphism and other life style parameters except for the association between Ser326Cys and smoking（P=0.027）.A significant increase of urinary 8-OHdG level was observed in Cys326Cys allelic healthy subjects（P=0.033）.Conclusion There are natural variations of hOGG1 gene in different ethnic populations in China.Smoking is related to the frequencies of Cys/Cys polymorphism,and the repairing of oxidative DNA damage is lower in individuals with the hOGG1 Cys326Cys genotype.

关 键 词：DNA修复 人8-羟基鸟嘌呤糖基酶(hOGG1) Ser326Cys多态性 生活方式 8-羟基-脱氧鸟嘌呤 

分 类 号：Q786[生物学—分子生物学]