氟化钠对小鼠睾丸间质瘤细胞StAR和P450scc mRNA表达的影响  

Effect of fluoride on the expression of StAR mRNA and P450scc mRNA in the testosterone synthesis of mouse leydig tumor cells

作　　者：管永波[1] 郝鹏飞[2] 唐春宇[1] 李苹[1] 李仕群[1] 程学敏[1] 巴月[1] 崔留欣[1] 

机构地区：[1]郑州大学公共卫生学院环境卫生学教研室,郑州450001 [2]新乡市疾病预防控制中心

出　　处：《卫生研究》2012年第1期105-108,共4页Journal of Hygiene Research

基　　金：2006年河南省卫生厅攻尖项目(No.20058010);2009年河南省卫生厅攻关项目(No.20093008)

摘　　要：目的观察氟化钠对小鼠睾丸间质瘤细胞（mLTC-1）中类固醇急性调节蛋白（StAR）和细胞色素P450胆固醇侧链裂解酶（P450scc）mRNA表达水平的影响。方法利用小鼠mLTC-1细胞为模型,用实时定量PCR法测定细胞中StAR和P450scc mRNA表达水平。采用放射免疫法测定细胞培养基上清中孕酮分泌量。结果与对照组比较,12、16和20μg/ml氟化钠浓度组的StAR和P450scc mRNA的表达量以及孕酮分泌量明显降低（P〈0.05）。结论氟化钠可以抑制mLTC-1细胞StAR和P450scc mRNA的表达,进而影响mLTC-1细胞孕酮的合成。Objective To observe the effect of sodium fluoride on the expression of steroidogenic acute regulatory protein（StAR） mRNA and cytochrome P450 cholesterol side-chain cleavage enzyme（P450scc） mRNA in the testosterone synthesis of mouse Leydig tumor cells（mLTC-1）.and to explore the molecular mechanisms of fluoride on reproductive injury.Methods Using mLTC-1 cells as a model,progesterone in the supernatant of cell culture medium was measured by RIA.The expression of StAR mRNA and P450scc mRNA in mLTC-1 cells was determined by real-time PCR.Results In comparison with the control group,the expression of StAR mRNA and P450scc mRNA in mLTC-1 cells and the secretion of progesterone of mLTC-1 cells in the three fluoride groups with 12,16 and 20 μg/ml of NaF in their media respectively were obviously lower（P0.05）.Conclusion The expression of StAR mRNA and P450scc mRNA in mLTC-1 cells could be inhibited by NaF,and consequently the secretion of progesterone in mLTC-1 cells was affected.

关 键 词：氟化钠 MA-10小鼠睾丸间质瘤细胞 类固醇急性调节蛋白 细胞色素P450胆固醇侧链裂解酶 

分 类 号：Q813[生物学—生物工程]