均匀设计法优选党参茯苓水溶性多糖的微波提取工艺  

Optimization of Microwave Extraction Technology for Water Soluble Polysaccharides from Codonopsis pilosula and Poria Cocos by Uniform Design Method

作　　者：王秀文[1] 王颖莉[1] 裴晓丽[1] 高宏[1] 

机构地区：[1]山西中医学院,太原030024

出　　处：《中国实验方剂学杂志》2012年第4期24-27,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省科技厅科技攻关项目(20100311090);太原市科技局项目(110148092)

摘　　要：目的:优选微波法提取党参、茯苓混合水溶性多糖的适宜条件。方法:采用均匀设计法考察微波功率、提取时间、料液比和提取次数4个因素对混合多糖含量的影响。结果:最佳微波提取工艺条件为微波功率130 W,提取时间6 min,料液比1∶40(g.mL-1),提取3次。微波混合多糖的提取率15.79%;传统混合多糖的提取率为15.11%。红外光谱图分析表明微波提取基本没有破坏多糖的结构。结论:微波提取的混合多糖含量和传统提取的混合多糖含量无显著性差异,但微波提取迅速、方法简便,适合工业化生产。Objective：To optimize microwave extraction conditions of mixed water-soluble from Codonopsis pilosula and Poria cocos.Method：Uniform design method was usd to investigate effects of microwave power,extraction time,ratio of solid-solvent and extraction times on the content of mixed polysaccharide.Result：Optimum microwave extraction technology conditions were as follows：microwave power was 130 W,extraction time was 6 min,ratio of solid-solvent was 1：40（g·mL-1）,extraction times was 3 times,yield of mixed water-soluble polysaccharide was 15.79% by microwave extraction,and it was 15.11% by conventional extraction method.Conclusion：Microwave extraction and conventional extraction of mixed polysaccharide showed no significant difference,but because of microwave extraction method was rapid,simple,it was suitable for modern production.

关 键 词：党参 茯苓 混合多糖 均匀设计法 微波 FTIR 

分 类 号：R283.6[医药卫生—中药学]