HPLC测定广西木蝴蝶中木蝴蝶苷B和黄芩苷的含量  

Determination of Baicalein-7-O-gentiobioside and Baicalin in Senmen Oroxyli from Guangxi Province by HPLC

作　　者：曹阳阳[1] 晏仁义[2] 杨滨[2] 黄璐琦[2] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2012年第4期82-84,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部重大新药创制(2009ZX09308-005)

摘　　要：目的:建立木蝴蝶药材中木蝴蝶苷B和黄芩苷同时定量的HPLC法。方法:采用Agilent Zorbax Extend-C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-甲醇-0.3%磷酸溶液梯度洗脱,流速1 mL.min-1,柱温30℃,检测波长280 nm。结果:木蝴蝶苷B和黄芩苷的质量在0.061～1.525μg(r=0.999 9)和0.276～6.900μg(r=1.000 0)与峰面积呈良好的线性关系,平均回收率(n=6)分别为98.06%,100.35%。结论:所建立的HPLC法可用于木蝴蝶药材中木蝴蝶苷B和黄芩苷的含量测定,为木蝴蝶药材的质量控制提供参考依据。Objective：To establish an HPLC method of determining baicalein-7-O-gentiobioside and baicalin in Senmen oroxyli simultaneously.Method：The separation was performed at 30 ℃ on a Zorbax Extend-C18 column（4.6 mm × 250 mm,5 μm） with acetonitrile-methanol-0.3% H3PO4as the mobile phase.The flow ratewas 1 mL.min-1.The detection wavelength was at 280 nm.Result：The linear rage of baicalein-7-O-gentiobioside and baicalin was 0.061-1.525 μg（r=0.999 9） and 0.276-6.900 μg（r=1.000 0）,respectively.The average recoveries（n=6） of baicalein-7-O-gentiobioside and baicalin were 98.06% and 100.35%,respectively.Conclution：The established method can be used for quantitative analysis of baicalein-7-O-gentiobioside and baicalin in Senmen oroxyli,which can provide a reference for quality control of this traditional Chinese medicine.

关 键 词：木蝴蝶 木蝴蝶苷B 黄芩苷 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]