醒脑静中各成分对栀子提取物中栀子苷大鼠鼻腔吸收的影响  

Effect of Components in Xingnaojing on Rat Nasal Absorption of Geniposide from Gardenia Extract

作　　者：杜守颖[1] 陆洋[1] 姚宗玲[1] 陈晓兰[1] 翟永松[1] 吴清[1] 王玥[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2012年第4期146-150,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："重大新药创制"科技重大专项(2009ZX09502-008);教育部博士点基金(20090013110007);国家自然科学基金面上项目(81073057);北京中医药大学自主选题项目(JYBZZ-JS021);国家自然科学基金青年基金项目(81102816)

摘　　要：目的:研究醒脑静中冰片(天然或人工)、人工麝香及合方后对栀子提取物大鼠鼻腔吸收的影响。方法:以体积校正法,采用改良的大鼠在体鼻灌流模型,研究与不同成分配伍后栀子苷的大鼠鼻腔吸收情况。结果:质量浓度为100,250,1 000 mg.L-1的栀子提取物溶液(分别含栀子苷36.8,92,368 mg.L-1)中栀子苷吸收速率常数K分别为(2.15±0.70)×10-3,(1.97±0.48)×10-3,(1.87±0.81)×10-3 min-1,栀子苷的鼻腔吸收符合一级速率过程,为被动扩散吸收。栀子提取物按组方配比分别与天然冰片(艾片)、人工冰片、人工麝香及合方配伍时栀子苷K值分别为单用栀子提取物组的1.4,1.7,1.1,1.3倍。大鼠鼻腔吸收系数与体外黏膜渗透系数有很好的相关性,KA=0.220 6KI+0.559(r=0.991 0)。结论:醒脑静组方配伍后可显著增加栀子苷的鼻腔吸收,方中冰片发挥了主要的促吸收作用。Objective：To study on effect of borneol（natural or synthetic）,synthetic musk and total prescription of Xingnaojing on nasal absorption of gardenia extract.Method：After combined with different components,nasal absorption in rats of geniposide was investigated with modified in situ nasal perfusion model by volume correction method.Result：Absorption coefficients（K） of geniposide（the content of it were 36.8,92,368 mg·L-1,respectively） in gardenia extract solution（concentration were 100,250,1 000 mg·L-1） were（2.15 ± 0.70）×10-3,（1.97 ± 0.48）×10-3,（1.87 ± 0.81）×10-3 min-1,respectively.Nasal absorption of geniposide in gardenia extract fitted first-order rate process and could be considered as passive diffusion absorption.When gardenia extract combined with borneol（natural or synthetic）,synthetic musk and in total prescription of Xingnaojing,K value of geniposide was 1.4,1.7,1.1,1.3 times to it of gardenia extract alone,respectively.Correlation between in situ nasal absorption coefficients and in vitro mucosa permeability coefficients was good,KA=0.220 6 KI＋0.559（r =0.991 0）.Conclusion：Nasal absorption of geniposide could be remarkably increased in Xingnaojing prescription and borneol played a major role in promoting absorption in this prescription.

关 键 词：醒脑静 栀子提取物 大鼠鼻腔吸收 人工冰片 人工麝香 

分 类 号：R283.6[医药卫生—中药学] R945[医药卫生—中医学]