基因枪共转化法获得玉米转基因植株的研究  

Study on the Development of Transgenic Maize Plants by Particle-bombardment Co-transformation

作　　者：郭嘉[1] 孙传波[1] 陶蕊[1] 李海华[1] 孟凡梅[1] 曲文利[1] 郝东云[1] 袁英[1] 

机构地区：[1]吉林省农业科学院生物技术研究中心,长春130033

出　　处：《玉米科学》2012年第1期44-47,共4页Journal of Maize Sciences

基　　金：养分高效利用转基因玉米新品种培育(2008ZX08003-005)

摘　　要：通过基因枪共转化方法将磷高效利用phy基因、钾高效利用AlHAK1基因和筛选标记bar基因等外源基因导入玉米自交系H99的1 500块愈伤组织中。经PCR分析,获得79株阳性植株,其中53株为AlHAK1阳性,49株为phy阳性,23株为AlHAK1和phy阳性,单基因转化率为5.27%,双基因转化率为1.53%,共转化整合比率达到29.11%。The phy gene,AlHAK1 gene and bar gene were transferred into 1 500 maize embryonic calli of maize inbred line H99 by particle-bombardment co-transformation.Seventy-nine positive plants were gained by PCR analysis,in which 53 plants showed AlHAK1 positive,49 plants showed phy positive,23 plants showed AlHAK1 and phy positive.The transformation frequency of single-gene and bi-genes was 5.27% and 1.53%,respectively.The co-transformation frequency reached about 29.11%.

关 键 词：玉米 自交系 基因枪 共转化 

分 类 号：S513.035[农业科学—作物学]