茅苍术愈伤组织诱导及其细胞悬浮培养研究  

Research on callus induction and cell suspension culture of Atractylodes lancea

作　　者：陶金华[1] 濮雪莲[1] 江曙[2] 

机构地区：[1]南通大学医学院,江苏南通226019 [2]南京中医药大学药学院,南京210046

出　　处：《广西植物》2012年第1期118-123,共6页Guihaia

基　　金：国家自然科学基金(81102743);江苏省高校自然科学基础研究项目(07KJD360164);江苏省中医药局项目(HL07076)~~

摘　　要：采用正交试验法研究了不同的植物生长调节剂对茅苍术叶柄、叶片和根茎愈伤组织诱导的影响,结果表明,不同外植体在各自的最佳培养条件下,叶柄、叶片和根茎愈伤组织的诱导率分别为99.0%、83.5%和71.5%,以叶柄的培养效果最好,其中2,4-D对茅苍术愈伤组织的诱导具有极显著的效果,在各种植物生长调节剂组合中,诱导叶柄愈伤组织形成的最佳组合为0.4mg·L-1NAA、4.0mg·L-12,4-D和0.4mg·L-1KT,培养20d左右,诱导率达到99.0%。此外,将茅苍术叶柄细胞悬浮培养至18d时,细胞量、多糖和苍术素的含量均达到最大值,分别为9.07g·L-1、15.68mg·L-1和19.62ug·L-1。Effect of different plant growth regulator on petiole,leaf and rhizome callus induction was studied by orthogonal design in this paper.It showed that petiole,leaf and rhizome callus induction rate were 99.0%、83.5% and 71.5% in their optimum culture conditions respectively.And Petiole was the most easy to form callus.There was a significant promotion effect of 2,4-D on the callus induction.The optimal composition for petiole callus induction was 0.4 mg·L-1NAA、4.0 mg·L-1 2,4-D and 0.4 mg·L-1 KT;and induction rate reached 99.0% when petiole being cultured for 20 d.In addition,when cell from petiole callus was cultured for 18 d,the maximum yields of cell biomass,polysaccharide and atractylodin were 9.07 g·L-1、15.68 mg·L-1 and 19.62 ug·L-1respectively.

关 键 词：茅苍术 愈伤组织 细胞悬浮培养 

分 类 号：Q945[生物学—植物学]