批量筛选水稻T-DNA插入突变体库获得生殖发育相关突变体  

Rice mutants of reproductive development obtained from screening the rice mutant library of T-DNA insertional mutagenesis

作　　者：裴荣[1] 陆展华[2] 姚家玲[1] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070 [2]华中农业大学园艺林学学院,武汉430070

出　　处：《华中农业大学学报》2012年第2期133-138,共6页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30971551)

摘　　要：针对花器官形态和种子发育突变表型对大型水稻T-DNA插入突变体库进行筛选,获得了大量突变体信息及材料,在9 760个突变体家系中筛选得到177个花器官形态和数量异常的突变家系,突变频率为1.81%;对9 760个家系中的3 432个家系筛选得到179个种子发育缺陷的突变家系,突变频率为5.22%。对所获得的270个突变家系进行了T-DNA插入的阳性检测,阳性率为64.8%。利用公共数据库RMD(Rice MutantDatabase,RMD)给定的侧翼序列,鉴定了其中1个结实率较低的突变体家系,表明其突变表型和T-DNA插入共分离,为深入研究该基因的功能提供了重要的遗传材料。To identify rice mutants of floral organ and seed development,a rice mutant library of T- DNA insertional mutagenesis constructed by the national key laboratory of crop genetic improvement at Huazhong Agricultural University was screened on large scale. A large number of useful information of mutants were got. Among 9 760 stable lines of phenotype variant, 177 lines showed morphology and number change of floral organ, with mutation frequency of 1. 81~ 179 lines from the 3 432 lines showed abnormal seed development,the percent of mutants was 5.22%. 270 lines of phenotype variant interested were obtained and 175 lines showed T-DNA insertion through PCR test,the positive rate was 64. 8% ,which will lay the foundation for cloning genes related with rice floral organ and seed develop- ment. One low seed-setting mutant was identified and confirmed to be cosegregated with its corresponding T-DNA flanking sequences tags, providing an important genetic resource for further studying the function of this gene.

关 键 词：水稻 T-DNA插入突变 突变表型 生殖发育 共分离 

分 类 号：S511.502.4[农业科学—作物学] Q78[生物学—分子生物学]