水稻脆性突变体nbc(t)的主要特性和脆性基因的初步定位  

Characterization and gene mapping of a brittle culm mutant nbc(t) in rice

作　　者：王川丽[1,2] 王令强[1,2] 牟同敏[1,2] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室 [2]华中农业大学国家植物基因研究中心(武汉),武汉430070

出　　处：《华中农业大学学报》2012年第2期159-164,共6页Journal of Huazhong Agricultural University

基　　金：国家高技术研究发展“863”计划(2010AA101304);国家自然科学基金项目(30921091)

摘　　要：对脆性突变体的表型、主要农艺性状和稻米品质进行了考查,并对脆性基因进行了初步定位。结果表明:脆茎水稻nbc(t)与野生亲本9311在株高等形态特征上无明显区别,仅在机械强度上有区别;nbc(t)的根、茎、叶、穗及种子都很脆,易折断,且表现全生育期脆性。脆茎突变体nbc(t)的主要农艺性状与9311相似,产量略低于9311,除了整精米率偏低以外,其他稻米品质性状与9311相似。脆茎突变体nbc(t)与广占63-4S所配组合的产量和稻米品质与扬两优6号相当。与9311相比,nbc(t)茎秆的抗折力相当,但抗张力明显降低,纤维素含量约降低17%。遗传分析表明,nbc(t)的脆茎特性可能由2个基因位点控制,初步定位在9号染色体上的2个区段,一个位于9号染色体上段SSR标记RM3700和RM24371之间,遗传距离分别为1.3cM和3.1cM;另一个位于9号染色体下段INDEL标记CL062和CL045外侧,遗传距离分别为1.6cM和6.0cM。The mutant nbc（t） was a brittle culm mutant of rice found in the offspring of 9311^2/ IRBB21. The all tissues of plant of mutant nbc（t） are brittle during the whole growth. The main agronomic characters of the brittle mutant were investigated and gene mapping of brittle trait was initially made. The results showed that the main agronomic traits of nbc（t） were as similar as the parent 9311 ex- cept that its yield was low. The rice quality of nbc（t） was also similar to 9311 except of head rice rate. The yield and rice quality of hybrid between Guangzhan 63-4S and nbc（t） were as same as that of Yangli- angyou 6. Comparing with 9311, the anti-booklet strength was similar, but stretching resistance was lower. The cellulose content in culm of nbc（t） was lower than that of 9311 by 17G. The genetic analysis showed that the brittle culm trait was controlled by two pairs of dominant genes, which were mapped on two loci of chromosome 9, namely,one locus was between the SSR marker RM3700 and RM24371 with the genetic distance of 1.3 cM and 3.1 cM ; another locus was outside of the INDEL marker CL062 and CL045 with the genetic distance of 1.6 cM and 6.0 cM.

关 键 词：水稻脆茎突变体 nbc(t) 农艺性状 稻米品质 脆性基因 基因定位 

分 类 号：S511.503[农业科学—作物学]