HMC毒素诱导玉米专效寄主原生质体细胞凋亡的检测  

Detection of apoptosis in host protoplasts induced by HMC-toxin

作　　者：马春红[1] 李秀丽[1,2] 张红心[3] 董文琦[4] 戴志刚 王立安[2] 贾银锁[1] 

机构地区：[1]河北省农林科学院遗传生理研究所/河北省植物转基因中心,石家庄050051 [2]河北师范大学生命科学学院,石家庄050016 [3]唐山师范学院生命科学系,唐山063000 [4]河北省农林科学院科技处,石家庄050051 [5]湖北省土壤肥料工作站,武汉430064

出　　处：《华中农业大学学报》2012年第2期199-203,共5页Journal of Huazhong Agricultural University

基　　金：农业部"948"项目(2011-G1-07;2008-Z20);科技部国际科技合作项目(2006DFB02480);河北省自然科学基金项目(C2011301010);河北省人才工程培养经费项目(冀人社字[2010]353号);河北省科学技术研究与发展计划项目(10393912D)

摘　　要：以2对同核异质玉米B37和Mo17叶片的原生质体为试材,并将原生质体随机分为4组,HMC毒素质量浓度分别为0、50、100、150μg/mL,处理时间分别为1、3、6h。采用吖啶橙(AO)与溴化乙啶(EB)荧光复染的方法进行检测,观察处理后原生质体的凋亡特征及细胞凋亡率的变化趋势。结果表明:HMC毒素处理玉米原生质体后发生细胞凋亡,并出现凋亡小体、染色质边集或环状染色质等凋亡特征;经不同浓度HMC毒素处理B37和Mo17玉米原生质体,CB37的最大凋亡率为10.80%,NB37为4.90%,CMo17为21.00%,NMo17为8.83%;HMC毒素对2种基因型的C、N细胞质所测结果一致,均为C细胞质对毒素敏感,先出现细胞凋亡,N细胞质出现细胞凋亡的时间相对较晚;C、N之间的细胞凋亡率差异显著,且前者高于后者。当毒素浓度不变时,细胞凋亡率随毒素处理时间的延长而递增;当处理时间不变时,细胞凋亡率随毒素浓度的增加而上升。Apoptosis in protoplasts from homokaryon maize B37（CB37,NB37） and Mo17 （CMol7, NMol7） inbred lines treated by HMC-toxin were studied. Homokaryon maize B37 and Mo17 inbred lines were tested and the protoplasts may be stained by AO and EB. The protoplasts were divided into 4 groups. 3 groups were treated by 0,50,100,150 μg/mL of HMC-toxin as the experimental groups, and the induced time were 1,3,6 h to study the feature of apoptotic and the rate of apoptosis. The apoptotic features such as apoptotic bodies, chromatin margination or ring chromatin were found after the treatment. When treated hy HMC-toxin with different concentrations, the apoptosis rates of CB37, NB37, CMo17 and NMol7 maize protoplasts were 10. 80%, 4. 90%, 21.00~, and 8. 83%. According to the above study,a conclusion can be reached as follows： apoptosis in protoplasts from homokaryon maize B37 were the same as that of Mo17 ; CB37 and CMo17 were more sensitive to HMC-toxin than NB37 and NMo17, respectively; when treated by the same concentration of HMC-toxin, the apoptosis rates of CB37 and CMo17 maize protoplasts were significantly higher than NB37 and NMo17 respectively; the rates of apoptosis were significantly different between C and N,and the former was significantly higher than the latter. In addition,the apoptosis rate increased when the treatment time and the concentration increased.

关 键 词：玉米小斑病菌C小种 毒素 原生质体 细胞凋亡 检测 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]