清醒Beagle犬呼吸和循环系统功能监测模型建立及生物学特性分析  

Establishment and parameter analysis of a system for simultaneously monitoring cardiovascular and respiratory function in conscious Beagle dogs

作　　者：刘雁[1] 阳海鹰[1] 王国强[1] 原野[1] 吴纯启[1] 廖明阳[1] 王全军[1] 关勇彪[1] 丁日高[1] 王青秀[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国新药杂志》2012年第2期134-137,169,共5页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2008ZX09305-003);北京市自然科学基金(7092079);北京市新药研发系统创新服务平台建设(Z08030203080818)

摘　　要：目的:应用植入式遥测技术,建立Beagle犬清醒动物呼吸和循环系统功能检测模型,应用遥测技术对Beagle犬生理参数进行长时程采集和分析,为临床前药物安全性评价试验提供重要的生理基础参考值。方法:通过外科手术,在Beagle犬体内埋植TL11M3-D70-PCTP遥测传感器,以DSI遥测生理信号采集系统连续记录并分析Beagle犬在无束缚条件下24 h生理信号,分析其昼夜节律,并以特非那定作为阳性药物进行验证。结果:成功建立了Beagle犬植入式清醒动物模型,Beagle犬的生理活动具有明显的昼夜节律性,早上09:00左右是多项生理指标的最高点,特非那定能够明显延长Beagle犬的QTc间期。结论:试验获得了本中心GLP试验室饲养条件下动物的基础值,可为其在药物临床前试验中的应用提供重要的参考数据。Objective： To establish the Beagle dog model with implanted telemetry transmitter,and analyze the parameters of cardiovascular and respiratory systems in the conscious Beagle dogs.Methods： We implanted the telemetry TL11M3-D70-PCTP transmitters to the dogs.The 24-h parameters of cardiovascular and respiratory systems in the conscious telemetered dogs were recorded and analyzed using Dataquest ART 4.8 system.Terfenadine 30 mg·kg-1 was used as a positive control.Results： Telemetry transmitters were successfully implanted.A circadian rhythm was obvious in the parameters from the conscious dogs;at 09：00AM,most parameters reached their peaks.Terfenadine prolonged QTc intervals in Beagle dogs.Conclusion： The conscious and telemetered Beagle dogs can be used for simultaneously assessing cardiovascular and respiratory changes for a longer term in the studies of safety pharmacology.

关 键 词：遥测技术 BEAGLE犬 呼吸和循环系统 

分 类 号：R965.2[医药卫生—药理学]