酿酒酵母组蛋白变体Htz1调控基因转录的作用机制  

Molecular Mechanisms of Histone Variant Htz1 Action in the Transcriptional Regulation in Saccharomyces cerevisiae

作　　者：陈慧颖[1] 张家瑱[2] 万亚坤[1] 

机构地区：[1]东南大学生命科学研究院"发育与疾病相关基因"教育部重点实验室,南京210000 [2]美国普度大学生物化学系

出　　处：《中国生物化学与分子生物学报》2012年第2期121-126,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.31071146);东南大学优秀青年教师(教学)科研计划资助项目(No.3231001201);东南大学大学生创新实践项目;江苏省自然科学基金项目(No.BK2011599)~~

摘　　要：在真核生物染色质中,作为核心组蛋白的H2A是构成核小体重要组分,其变体之一H2A.Z高度保守,对真核细胞生物的生命活动有重要意义.模式生物酿酒酵母中的H2A.Z被称作Htz1.在对多种生物H2A.Z的研究中,以对酿酒酵母组蛋白变体Htz1的探讨最为深入全面.本文将从多个方面详细介绍酿酒酵母Htz1对基因表达调控的作用机制,涵盖其蛋白结构、染色质上的定位、翻译后修饰、结合机制、生物功能及其分子伴侣的作用等,并对未来该领域需要解决的重要科学问题进行了展望.As one member of four core histones,H2A plays an important role in setting up the nucleosome in eukaryotic chromatin.One of its variants is H2A.Z.H2A.Z is highly conserved and of essence for viability in high eukaryotic cells.Among extensive studies in several model organisms,significant progresses have been achieved by studying Htz1,the H2A.Z equivalent in Saccharomyces cerevisiae.This review focuses on several aspects of Htz1 in S.cerevisiae,involving its protein structure,mechanisms of deposition within the chromatin,post-translational modifications,molecular mechanisms of incorporation,biological functions and roles of histone variant associated chaperones.Meanwhile,some significant biological views in this field are discussed and anticipated as well.

关 键 词：酿酒酵母组蛋白变体Htz1 表观遗传学 转录调控 分子伴侣 

分 类 号：Q498.4[生物学—生理学]