耐亚胺培南鲍曼不动杆菌碳青霉烯酶检测及同源性分析  

Analysis of carbapenemases genotypes and homology of imipenem-resistant Acinetobacter baumanii

作　　者：芮晓艳[1,2] 胡杰贵[1] 熊自忠[3] 

机构地区：[1]安徽医科大学第一附属医院呼吸内科,合肥230022 [2]滁州市第二人民医院呼吸内科,滁州239001 [3]安徽医科大学第一附属医院感染科,合肥230022

出　　处：《安徽医科大学学报》2012年第2期154-157,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的了解临床分离亚胺培南耐药鲍曼不动杆菌(IRAB)碳青霉烯酶的基因型及同源性。方法采用Mi-croScan WalkAway-40微生物分析仪进行菌株鉴定和药敏试验,聚合酶链反应(PCR)检测OXA-23、OXA-24、OXA-51、OXA-58组碳青霉烯酶基因型,肠杆菌科重复序列PCR(ERIC-PCR)检测耐药基因阳性菌株的同源性。结果 64株IRAB均为多重耐药菌株,其中10株(15.6%)为泛耐药菌株,51株(79.7%)blaOXA-23阳性,4株(6.3%)blaOXA-58阳性,57株(89.1%)blaOXA-66/blaOXA-51组阳性。ERIC-PCR结果显示blaOXA-23阳性菌株中47株为同一基因谱。结论产OXA-23型碳青霉烯酶是我院鲍曼不动杆菌对亚胺培南耐药的重要机制,菌株间可能存在克隆传播。Objective To investigate the carbapenemases genotypes and homology of imipenem-resistant Acinetobacter baumanii（IRAB）.Methods MicroScan WalkAway-40 automatic microbial analysis system was applied to bacilli identification and antimicrobial susceptibility testing.Polymerase chain reaction（PCR） assay was performed for the detection of genes encoding for OXA-23-like,OXA-24-like,OXA-51-like,OXA-58-like carbapenemase,and enterobacterial repetitive consensus（ERIC-PCR） was performed for stain identification.Results All of IRAB were multidrug-resistant,10 strains（51.6%） were pan-resistant.51 strains（79.7%） were blaOXA-23 positive,and 4 strains（6.3%） were blaOXA-58 positive,57 strains（89.1%） were blaOXA-66/ blaOXA-51 positive.ERIC-PCR showed that 47 strains blaOXA-23 positive had the same genetic spectrum.Conclusion OXA-23-like carbapenemase in Acinetobacter baumanii is the most prevalent enzyme in imipenem resistant isolates.Clone dissemination may be the most important way of strains spread.

关 键 词：鲍曼不动杆菌 碳青霉烯酶 同源性 

分 类 号：R378.99[医药卫生—病原生物学] R978.11[医药卫生—基础医学]